caa a22 4500 475794451 CHVBK 20180406123719.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001220051520 doi (NATIONALLICENCE)springer-10.1007/s001220051520 RFLP and RAPD mapping in flax ( Linum usitatissimum ) [Elektronische Daten] [T. J. Oh, M. Gorman, C. A. Cullis] Abstract : A map of flax (Linum usitatissimum) using restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs), and comprising 15 linkage groups containing 94 markers, has been developed covering about 1000 cM. The mapping populations were the F2 populations from two crosses between diverse cultivars. From one cross, CI1303 and Stormont Cirrus, 20 RFLP and 520 RAPD markers were analyzed. Thirteen RFLP and 80 RAPD markers were on the 15 linkage groups, in addition to one sequence-tagged site (STS). Seven polymorphic RAPD markers were found to have unusual segregation patterns. RAPDs were expressed as dominant markers, but for these markers a prevalence of the progeny lacked a band rather than the expected one-fourth ratio. However, these exceptions may be related to the instability of the genome of Stormont Cirrus in which stable and heritable genomic changes can be induced by environmental factors. The current map could be used for the identification of markers linked to loci controlling the ability to generate heritable changes in response to environmental growth conditions, and to develop anchor loci with STSs for a more general application. Springer-Verlag Berlin Heidelberg, 2000 Key words RFLPs nationallicence RAPDs nationallicence Flax nationallicence Linkage map nationallicence Oh T. J. Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USA e-mail: cac5@po.cwru.edu, US aut Gorman M. Biology Department, Baldwin Wallace College, Berea, OH 44107, USA, US aut Cullis C. A. Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USA e-mail: cac5@po.cwru.edu, US aut https://doi.org/10.1007/s001220051520 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051520 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oh T. J. Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USA e-mail: cac5@po.cwru.edu, US aut NATIONALLICENCE 700 1- Gorman M. Biology Department, Baldwin Wallace College, Berea, OH 44107, USA, US aut NATIONALLICENCE 700 1- Cullis C. A. Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USA e-mail: cac5@po.cwru.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer