caa a22 4500 475794508 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001220051511 doi (NATIONALLICENCE)springer-10.1007/s001220051511 Soybean Kunitz, C-II and PI-IV inhibitor genes confer different levels of insect resistance to tobacco and potato transgenic plants [Elektronische Daten] [S. Marchetti, M. Delledonne, C. Fogher, C. Chiabà, F. Chiesa, F. Savazzini, A. Giordano] Abstract : In modern, highly intensive agriculture, the control of insect pests is basically achieved with the application of chemical pesticides. Heavy reliance on this sole strategy is associated with several drawbacks, and the development of alternative or complementary methods to chemical control is desirable. In this work, three soybean genes (KTi 3 , C-II and PI-IV)coding for serine proteinase inhibitors were isolated by PCR and transferred to Agrobacterium tumefaciens EHA 105, which in turn was used for transforming tobacco leaf and potato tuber discs. Biochemical assays confirmed that transgenic plants synthesized serine proteinase inhibitors; rates of expression varied among plants. The level of insect resistance (tested with Spodoptera littoralis Boisduval) was particularly high in tobacco, where many plants caused the death of all larvae. In potatoes, larval mortality was much less frequently achieved, but the results were still encouraging in that larval weight gain was reduced by 50% in the presence of adequate amounts of inhibitor. When 8-day-old larvae were fed different KTi 3 -expressing tobacco plants, a highly significant (P<0.01) correlation was observed between inhibitor content and larval live weight. Larval weight gain was found to be dependent on midgut proteolytic activity. On the basis of the evidence collected, it is suggested that further work is required to identify more specific inhibitors for the main proteinases of the target insect. Springer-Verlag Berlin Heidelberg, 2000 Key words Insect resistance nationallicence Tobacco nationallicence Potato nationallicence Soybean Kunitz and Bowman-Birk inhibitor genes nationallicence Marchetti S. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut Delledonne M. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut Fogher C. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut Chiabà C. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut Chiesa F. Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy, IT aut Savazzini F. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut Giordano A. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut https://doi.org/10.1007/s001220051511 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051511 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marchetti S. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut NATIONALLICENCE 700 1- Delledonne M. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut NATIONALLICENCE 700 1- Fogher C. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut NATIONALLICENCE 700 1- Chiabà C. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut NATIONALLICENCE 700 1- Chiesa F. Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy, IT aut NATIONALLICENCE 700 1- Savazzini F. Istituto di Botanica e Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, IT aut NATIONALLICENCE 700 1- Giordano A. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via delle Scienze 208, 33100 Udine, Italy Fax: +39 0432-558603, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer