caa a22 4500 475794532 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051449 doi (NATIONALLICENCE)springer-10.1007/s001220051449 Fine physical mapping of the rice stripe resistance gene locus, Stvb-i [Elektronische Daten] [Y. Hayano-Saito, K. Saito, S. Nakamura, S. Kawasaki, M. Iwasaki] Abstract : The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones. Springer-Verlag Berlin Heidelberg, 2000 Keywords Rice nationallicence Stripe disease resistance gene nationallicence Stvb-i nationallicence BAC nationallicence Physical map nationallicence Hayano-Saito Y. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut Saito K. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut Nakamura S. Tohoku National Agricultural Experimental Station, Shimokuriyagawa, Morioka, Iwate 020-0123, Japan, JP aut Kawasaki S. National Institute of Agrobiological Resources (NIAR), Kan-nondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan, JP aut Iwasaki M. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut https://doi.org/10.1007/s001220051449 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051449 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayano-Saito Y. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut NATIONALLICENCE 700 1- Saito K. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut NATIONALLICENCE 700 1- Nakamura S. Tohoku National Agricultural Experimental Station, Shimokuriyagawa, Morioka, Iwate 020-0123, Japan, JP aut NATIONALLICENCE 700 1- Kawasaki S. National Institute of Agrobiological Resources (NIAR), Kan-nondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan, JP aut NATIONALLICENCE 700 1- Iwasaki M. Hokkaido National Agricultural Experiment Station (HNAES), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan e-mail: hsyuriko@cryo.affrc.go.jp, Tel.: +81-11-851-9578, Fax: +81-11-851-2178, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer