caa a22 4500 475794605 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051464 doi (NATIONALLICENCE)springer-10.1007/s001220051464 Onion microsatellites for germplasm analysis and their use in assessing intra- and interspecific relatedness within the subgenus Rhizirideum [Elektronische Daten] [D. Fischer, K. Bachmann] Abstract : We have identified a set of informative STMS markers in onion (Allium cepa L.) and report on their application for genotyping and for determining genetic relationships. The markers have been developed from a genomic library enriched for microsatellites. Integrity of the microsatellite polymorphism was confirmed by amplicon sequencing. The microsatellite genotypes of 83 onion accessions and landraces from living onion collections were compared. As few as four primer pairs were sufficient to assign unique microsatellite patterns to the 83 accessions. Some of the microsatellite markers can be used for interspecific taxonomic analyses among close relatives of Allium cepa. Generally, our data support and extend results obtained from recently performed analyses using ITS, RAPD and morphology. Springer-Verlag Berlin Heidelberg, 2000 Key words Allium nationallicence Onion nationallicence Genotyping nationallicence Germplasm analysis nationallicence Microsatellite nationallicence Simple sequence repeat nationallicence Fischer D. Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany, e-mail: bachmann@ipk-gatersleben.de, URL: http://www.ipk-gatersleben.de/fischerd/tag/, Fax: +49-039482-5155, DE aut Bachmann K. Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany, e-mail: bachmann@ipk-gatersleben.de, URL: http://www.ipk-gatersleben.de/fischerd/tag/, Fax: +49-039482-5155, DE aut https://doi.org/10.1007/s001220051464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fischer D. Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany, e-mail: bachmann@ipk-gatersleben.de, URL: http://www.ipk-gatersleben.de/fischerd/tag/, Fax: +49-039482-5155, DE aut NATIONALLICENCE 700 1- Bachmann K. Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany, e-mail: bachmann@ipk-gatersleben.de, URL: http://www.ipk-gatersleben.de/fischerd/tag/, Fax: +49-039482-5155, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer