caa a22 4500 475794656 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051452 doi (NATIONALLICENCE)springer-10.1007/s001220051452 Physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oleracea [Elektronische Daten] [G. Suzuki, M. Watanabe, T. Nishio] Abstract : In Brassica, self-incompatibility genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are located in the S-locus complex region with several other S-linked genes. The S locus is a highly polymorphic region: polymorphism has been observed not only in sequences of SLG and SRK but also in the location of the S-locus genes. In order to compare the physical location of the S-locus genes in various S haplotypes, we used six class-I S haplotypes of B. rapa and seven class-I S haplotypes of B. oleracea in this study. DNA gel blot analysis using pulsed-field gel electrophoresis (PFGE) showed that the physical distances between SLG and SRK in B. rapa are significantly shorter than those in B. oleracea and that the sizes of MluI and BssHII fragments harboring SLG and SRK are less variable within B. rapa than within B. oleracea. We concluded that several large genomic fragments might have been inserted into the S-locus region of B. oleracea after allelic differentiation of S-locus genes. Springer-Verlag Berlin Heidelberg, 2000 Key words Brassica rapa (syn. campestris) nationallicence B. oleracea nationallicence PFGE nationallicence S-locus nationallicence Self-incompatibility nationallicence Suzuki G. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut Watanabe M. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut Nishio T. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut https://doi.org/10.1007/s001220051452 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051452 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki G. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut NATIONALLICENCE 700 1- Watanabe M. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut NATIONALLICENCE 700 1- Nishio T. Laboratory of Plant Breeding, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan Fax: 81-22-717-8654 e-mail: nishio@bios.tohoku.ac.jp, Present addresses: G. Suzuki, Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan M. Watanabe, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer