caa a22 4500 475794680 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051443 doi (NATIONALLICENCE)springer-10.1007/s001220051443 Field performance of Xa21 transgenic indica rice (Oryza sativa L.), IR72 [Elektronische Daten] [J. Tu, K. Datta, G. S. Khush, Q. Zhang, S.K. Datta] Abstract : Based on the characterization of the resistance phenotype and molecular analysis, several homozygous lines carrying Xa21 against the bacterial blight (BB) pathogen were obtained from previously transformed indica rice, IR72. The homozygous line, T103-10, with the best phenotype and seed-setting, was repeatedly tested under normal field conditions to evaluate its levels of resistance to the BB pathogen in Wuhan, China, in 1998 and 1999. The isolates of Xanthomonas oryzae pv oryzae (Xoo) used in this experiments were PXO61, PXO79, PXO99 and PXO112 isolated from the Philippines, T2 isolated from Japan, and Zhe173 isolated from China. The results demonstrated that the transgenic homozygous line expressed the same resistance spectrum, but with a shorter lesion length to each inoculated isolates as the lesion length of the Xa21 donor line IRBB21. The non-transformed control IR72 carrying Xa4 was resistant to PXO61, PXO112, Zhe173 and T2, but susceptible to PXO99 and PXO79. The negative control variety IR24 was susceptible to all isolates under field conditions. The results demonstrated clearly that the Xa21 transgene led to an excellent field performance of the introduced bacterial blight resistance trait on the recipient plants. The yield performance of this transgenic homozygous line, T103-10, is comparable with that of the control under field conditions. Springer-Verlag Berlin Heidelberg, 2000 Key words Xa21 nationallicence IR72 nationallicence Bacterial leaf blight nationallicence Transformation nationallicence Field testing nationallicence Tu J. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut Datta K. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut Khush G. S. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut Zhang Q. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China, CN aut Datta S.K. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut https://doi.org/10.1007/s001220051443 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051443 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tu J. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut NATIONALLICENCE 700 1- Datta K. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut NATIONALLICENCE 700 1- Khush G. S. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut NATIONALLICENCE 700 1- Zhang Q. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China, CN aut NATIONALLICENCE 700 1- Datta S.K. Plant Breeding, Genetics, and Biochemistry Division, International Rice Research Institute, MCPO Box 3127, 1271 Makati Ciyt, The Philippines e-mail: SDATTA@cgiar.org, Tel.: +63-2-8450563, -8127686, Fax: +63-2-8911292, -8178470, XX aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer