caa a22 4500 475794699 CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051456 doi (NATIONALLICENCE)springer-10.1007/s001220051456 Microsatellite analysis of Aegilops tauschii germplasm [Elektronische Daten] [E. Pestsova, V. Korzun, N. P. Goncharov, K. Hammer, M.W. Ganal, M.S. Röder] Abstract : The highly polymorphic diploid grass Aegilops tauschii isthe D-genome donor to hexaploid wheat and represents a potential source for bread wheat improvement. In the present study microsatellite markers were used for germplasm analysis and estimation of the genetic relationship between 113 accessions of Ae. tauschii from the gene bank collection at IPK, Gatersleben. Eighteen microsatellite markers, developed from Triticum aestivum and Ae. tauschii sequences, were selected for the analysis. All microsatellite markers showed a high level of polymorphism. The number of alleles per microsatellite marker varied from 11 to 25 and a total of 338 alleles were detected. The number of alleles per locus in cultivated bread wheat germplasm had previously been found to be significantly lower. The highest levels of genetic diversity for microsatellite markers were found in accessions from the Caucasian countries (Georgia, Armenia and the Daghestan region of Russia) and the lowest in accessions from the Central Asian countries (Uzbekistan and Turkmenistan). Genetic dissimilarity values between accessions were used to produce a dendrogram of the relationships among the accessions. The result showed that all of the accessions could be distinguished and clustered into two large groups in accordance with their subspecies taxonomic classification. The pattern of clustering of the Ae. tauschii accessions is according to their geographic distribution. The data suggest that a relatively small number of microsatellites can be used to estimate genetic diversity in the germplasm of Ae. tauschii and confirm the good suitability of microsatellite markers for the analysis of germplasm collections. Springer-Verlag Berlin Heidelberg, 2000 Key words Aegilops tauschii nationallicence D genome nationallicence Microsatellite markers nationallicence Genetic diversity nationallicence Germplasm nationallicence Pestsova E. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de Tel: +49-39482 5511, Fax: +49-39482 5137, DE aut Korzun V. aut Goncharov N. P. Institute of Cytology and Genetics, Lavrentiev Ave. 10, Novosibirsk, 630090, Russia, RU aut Hammer K. Universität Gesamthochschule Kassel, Steinstrasse 11, 37213 Witzenhausen, Germany, DE aut Ganal M.W. aut Röder M.S. aut https://doi.org/10.1007/s001220051456 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051456 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pestsova E. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de Tel: +49-39482 5511, Fax: +49-39482 5137, DE aut NATIONALLICENCE 700 1- Korzun V. aut NATIONALLICENCE 700 1- Goncharov N. P. Institute of Cytology and Genetics, Lavrentiev Ave. 10, Novosibirsk, 630090, Russia, RU aut NATIONALLICENCE 700 1- Hammer K. Universität Gesamthochschule Kassel, Steinstrasse 11, 37213 Witzenhausen, Germany, DE aut NATIONALLICENCE 700 1- Ganal M.W. aut NATIONALLICENCE 700 1- Röder M.S. aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer