caa a22 4500 47579477X CHVBK 20180406123720.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051460 doi (NATIONALLICENCE)springer-10.1007/s001220051460 Linkage analysis of anther-derived monoploids showing distorted segregation of molecular markers [Elektronische Daten] [G. C. C. Tai, J. E. A. Seabrook, A. N. Aziz] Abstract : Monoploids can be obtained from several diploid plant species by anther culture. Mapping of molecular markers using monoploids is greatly facilitated by the simple 1:1 segregation ratio expected from all heterozygous loci in the genome. Distorted segregation of molecular markers, however, appears to be a common phenomenon in many crop species and hinders the use of monoploids for mapping purposes. This report examines the segregation pattern of two marker genes linked together with one locus or separately with two independent loci which are responsible for the observed distortion. Each of the loci exhibiting distorted segregation has one of the two alleles which inhibits regeneration of the gametic cells in vitro and disrupts the expected segregation ratio of the linked markers. All possible situations in which linkage occurs between markers and distortion-causing genes are considered. Theoretical results outlining the segregation pattern among these linkage types indicate that the distinguishable distorted ratios can be used for mapping purposes. A protocol is given for the mapping of distorted gene markers based on existing gene mapping software. An example is presented of the mapping of distorted RAPD markers of monoploids obtained from a diploid potato genotype. Springer-Verlag Berlin Heidelberg, 2000 Key words Gene mapping nationallicence Potato nationallicence Segregation ratio nationallicence Solanum tuberosum L nationallicence Tai G. C. C. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut Seabrook J. E. A. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut Aziz A. N. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut https://doi.org/10.1007/s001220051460 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051460 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tai G. C. C. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut NATIONALLICENCE 700 1- Seabrook J. E. A. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut NATIONALLICENCE 700 1- Aziz A. N. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, N.B., Canada, E3B 4Z7 e-mail: taig@em.agr.ca; seabrookj@em.agr.ca, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer