caa a22 4500 475794826 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051475 doi (NATIONALLICENCE)springer-10.1007/s001220051475 Characterization and linkage mapping of R-gene analogous DNA sequences in pea (Pisum sativum L.) [Elektronische Daten] [G. M. Timmerman-Vaughan, T. J. Frew, N. F. Weeden] Abstract : Pea (Pisum sativum L.) sequences that are analogous to the conserved nucleotide binding site (NBS) domain found in a number of plant disease resistance genes (R-genes) were cloned. Using redundant oligonucleotide primers and the polymerase chain reaction (PCR), we amplified nine pea sequences and characterised their sequences. The pea R-gene analog (RGA)- deduced amino acid sequences demonstrated significant sequence similarity with known R-gene sequences lodged in public databases. The genomic locations of eight of the pea RGAs were determined by linkage mapping. The eight RGAs identified ten loci that mapped to six linkage groups. In addition, the genomic organization of the RGAs was inferred. Both single-copy and multicopy sequence families were present among the RGAs, and the multicopy families occurred most often as tightly linked clusters of related sequences. Intraspecific copy number variability was observed in three of the RGA sequence families, suggesting that these sequence families are evolving rapidly. The genomic locations of the pea RGAs were compared with the locations of known pea R-genes and sym genes involved in the pea-rhizobia symbiosis. Two pea RGAs mapped in the genomic region containing a pea R-gene, Fw, and four pea RGAs mapped in regions of the genome containing sym genes. Springer-Verlag Berlin Heidelberg, 2000 Key words NBS-LRR nationallicence Disease resistance nationallicence sym genes nationallicence Legume-rhizobia symbiosis nationallicence Timmerman-Vaughan G. M. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut Frew T. J. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut Weeden N. F. Department of Horticultural Science, Cornell University, Geneva NY 14456, USA, Present address: Department of Plant Sciences, Montana State University, Bozeman MT 59717-7622, USA, US aut https://doi.org/10.1007/s001220051475 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051475 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Timmerman-Vaughan G. M. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut NATIONALLICENCE 700 1- Frew T. J. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut NATIONALLICENCE 700 1- Weeden N. F. Department of Horticultural Science, Cornell University, Geneva NY 14456, USA, Present address: Department of Plant Sciences, Montana State University, Bozeman MT 59717-7622, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer