caa a22 4500 475794834 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051479 doi (NATIONALLICENCE)springer-10.1007/s001220051479 Conservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer aerietinum L.) within the genus Cicer [Elektronische Daten] [W. Choumane, P. Winter, F. Weigand, G. Kahl] Abstract : The conservation of 90 microsatellite-flanking sequences from chickpea in 39 accessions of eight annual and 1 accession of a perennial species of the genus Cicer was investigated. All of the primer sequences successfully amplified microsatellites in related species, indicating the conservation of microsatellite-flanking sequences in chickpea's relatives. However, the degree of conservation of the primer sites varied between species depending on their known phylogenetic relationship to chickpea, ranging from 92.2% in C. reticulatum, chickpea's closest relative and potential ancestor, down to 50% for C. cuneatum. A phylogenetic tree revealed that chickpea and the other members of its crossability group were more closely related to the perennial C. anatolicum than to other annual species of the genus. Considerable variation in size and number of amplification products between and within species was observed. Sequence analysis of highly divergent amplification products proved that variation is either due to large differences in the number of microsatellite repeats or to the amplification of a locus unrelated to the one amplified from chickpea. Sequence information and bootstrapping using PAUP suggested that STMSs derived from chickpea may be efficiently and reliably used for synteny studies in chickpea's crossability group, including C. anatolicum. However, care should be taken when applying these markers to other species of the genus. Considering the data presented here and the known historical record, the age of section Monocicer, including chickpea, is estimated to be about 100,000 years. Springer-Verlag Berlin Heidelberg, 2000 Key words Cicer species nationallicence Microsatellites nationallicence Zero-allele nationallicence Phylogenetic tree nationallicence Evolution nationallicence Choumane W. International Centre for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria, and Faculty of Agriculture, University of Tishreen, Lattakia, Syria, XX aut Winter P. Plant Molecular Biology, Biocentre, University of Frankfurt/Main, D-60439 Frankfurt, Germany e-mail: P.winter@em.uni-frankfurt.de, DE aut Weigand F. ICARDA, P.O. Box 5466, Aleppo, Syria, XX aut Kahl G. Plant Molecular Biology, Biocentre, University of Frankfurt/Main, D-60439 Frankfurt, Germany e-mail: P.winter@em.uni-frankfurt.de, DE aut https://doi.org/10.1007/s001220051479 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051479 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choumane W. International Centre for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria, and Faculty of Agriculture, University of Tishreen, Lattakia, Syria, XX aut NATIONALLICENCE 700 1- Winter P. Plant Molecular Biology, Biocentre, University of Frankfurt/Main, D-60439 Frankfurt, Germany e-mail: P.winter@em.uni-frankfurt.de, DE aut NATIONALLICENCE 700 1- Weigand F. ICARDA, P.O. Box 5466, Aleppo, Syria, XX aut NATIONALLICENCE 700 1- Kahl G. Plant Molecular Biology, Biocentre, University of Frankfurt/Main, D-60439 Frankfurt, Germany e-mail: P.winter@em.uni-frankfurt.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer