caa a22 4500 475794850 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051483 doi (NATIONALLICENCE)springer-10.1007/s001220051483 Conservation of microsatellite loci within the genus Vitis [Elektronische Daten] [G. Di Gaspero, E. Peterlunger, R. Testolin, K. J. Edwards, G. Cipriani] Abstract : Eleven microsatellites isolated from grapevine (Vitis vinifera) were used to study the degree of conservation of these sequences across different Vitis species. Nine microsatellites were newly isolated, the remaining two (VVS2 and VVS5) came from the literature. A preliminary assay on the conservation of priming sites was carried out on 14 non-V. vinifera species, including relevant taxa for breeding. Parthenocissus quinquefolia was added as representative of a related genus. Cross-species amplification was obtained in 94% of the 176 genotype×locus tested combinations. Three microsatellite loci were then cloned and sequenced in ten species. The microsatellite repeat was found present in all cases. The repeat region was often longer in V. vinifera than in the other species. Furthermore the non-source species showed interruptions in the repeat. In spite of these constraints, which could reduce the polymorphism of microsatellites in non-source species, the results demonstrate the possibility of extending the use of microsatellite markers to wild germplasm and inter-specific hybrids. Point mutations have been found in microsatellite flanking regions and these variations have been used to investigate the genetic relationship among taxa. The Neighbor-joining tree that was obtained on the basis of ten nucleotide variations, showed that there is not a clear cut difference between American, Asian and European species and that the actual taxonomy which reflects the geographical distribution of species must most likely be revised. Moreover, in general, nucleotide variations which occur in microsatellite flanking regions provide new molecular tools for investigating the evolution of species. Springer-Verlag Berlin Heidelberg, 2000 Key words Simple sequence repeats nationallicence Inter-specific priming nationallicence Molecular markers nationallicence Grapevine nationallicence Di Gaspero G. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut Peterlunger E. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut Testolin R. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut Edwards K. J. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, UK, GB aut Cipriani G. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut https://doi.org/10.1007/s001220051483 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051483 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Gaspero G. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut NATIONALLICENCE 700 1- Peterlunger E. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut NATIONALLICENCE 700 1- Testolin R. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut NATIONALLICENCE 700 1- Edwards K. J. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, UK, GB aut NATIONALLICENCE 700 1- Cipriani G. Dipartimento di Produzione Vegetale e Tecnologie Agrarie, University of Udine, via delle Scienze, 208-33100 Udine, Italy e-mail: guido.cipriani@dpvta.uniud.it Tel.: +39-0432-558635, Fax: +39-0432-558603, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer