caa a22 4500 475794877 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051485 doi (NATIONALLICENCE)springer-10.1007/s001220051485 Development and characterization of microsatellite markers in black poplar (Populus nigra L.) [Elektronische Daten] [J. van der Schoot, M. Pospíšková, B. Vosman, M. J. M. Smulders] Abstract : Using an enrichment procedure, we have cloned and sequenced microsatellite loci from black poplar (Populus nigra L.) and developed primers for sequence-tagged microsatellite (STMS) analysis. Twelve primer pairs for dinucleotide repeats produced fragments of sufficient quality which were polymorphic in P. nigra. Some of them also showed amplification in other Populus species (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, and/or P. lasiocarpa). The best nine and (GT) (GA) microsatellite markers were tested on a set of 23 P. nigra genotypes from all over Europe. The microsatellites were highly polymorphic, with 10-19 different alleles per microsatellite locus among these 23 genotypes. WPMS08 sometimes amplified three fragments. Using the other eight marker loci, the level of heterozygosity among the plants was on average 0.71 (range 0.25-1.00). The microsatellite markers developed will be useful for screening the genetic diversity in natural populations and in gene bank collections. Springer-Verlag Berlin Heidelberg, 2000 Key words SSR nationallicence Genetic diversity nationallicence Dinucleotide repeat nationallicence van der Schoot J. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut Pospíšková M. Research Institute of Ornamental Gardening, 25243 Pruhonice, Czech Republic, XX aut Vosman B. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut Smulders M. J. M. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut https://doi.org/10.1007/s001220051485 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051485 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van der Schoot J. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut NATIONALLICENCE 700 1- Pospíšková M. Research Institute of Ornamental Gardening, 25243 Pruhonice, Czech Republic, XX aut NATIONALLICENCE 700 1- Vosman B. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut NATIONALLICENCE 700 1- Smulders M. J. M. Plant Research International, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: m.j.m.smulders@plant.wag-ur.nl Fax: +31-317 418094, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer