caa a22 4500 475794893 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051446 doi (NATIONALLICENCE)springer-10.1007/s001220051446 Transposition of the En/Spm transposable element system in maize (Zea mays L.): reciprocal crosses of a1-m(Au) and a1-m(r) alleles uncover developmental patterns [Elektronische Daten] [B.-S. Seo, P. A. Peterson] Abstract : Transposition studies of the transposon, En/Spm, have dealt with general aspects of the timing of the excision event with regard to DNA replication and plant development, but without describing details of the process. By following the excision events of an En transposon inserted at the a1 locus [a1-m(Au)], several features of this process can be elucidated. In progenies from reciprocal crosses between the a1-m(Au) allele containing an En insert, and a nonautonomous En allele, [a1-m(r) is a deficiency derivative of En], several features of the En at the a1-m(Au) allele can be observed taking place during ear development and during microsporogenesis. First, it has long been known that the distribution of mutant kernel phenotypes on an ear indicates that En transposes late in most of the events during ear development. Second, the phase change of En (presence and absence of activity) is observed during cob development. Third, discordant kernel phenotypes of two ears, reported herein, resulting from a reciprocal cross with the parental phenotype can be deduced to arise from the transposition of En during microsporogenesis and subsequent fertilization, leading to a discordant genotype between endosperm and embryo. The phase change and discordance lead us to conclude that these events can arise from transposition after host DNA replication. It can also be concluded that the activity of the En inserted in this a1-m(Au) allele is not limited to a specific stage or timing during plant development. Further, this study illustrates the power of genetic analysis in the determination of cellular events. Springer-Verlag Berlin Heidelberg, 2000 Key words Transposable element En/Spm nationallicence Reciprocal cross nationallicence Transposition during plant development nationallicence Discordance nationallicence Seo B.-S Department of Agronomy, G407 Agronomy Hall, Iowa State University, Ames, Iowa 50011, USA e-mail: pap@iastate.edu Tel.: +1-515-294-9652, Fax: +1-515-294-2299, Present address: B.-S. Seo, Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, USA, US aut Peterson P. A. Department of Agronomy, G407 Agronomy Hall, Iowa State University, Ames, Iowa 50011, USA e-mail: pap@iastate.edu Tel.: +1-515-294-9652, Fax: +1-515-294-2299, Present address: B.-S. Seo, Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, USA, US aut https://doi.org/10.1007/s001220051446 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051446 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seo B.-S Department of Agronomy, G407 Agronomy Hall, Iowa State University, Ames, Iowa 50011, USA e-mail: pap@iastate.edu Tel.: +1-515-294-9652, Fax: +1-515-294-2299, Present address: B.-S. Seo, Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, USA, US aut NATIONALLICENCE 700 1- Peterson P. A. Department of Agronomy, G407 Agronomy Hall, Iowa State University, Ames, Iowa 50011, USA e-mail: pap@iastate.edu Tel.: +1-515-294-9652, Fax: +1-515-294-2299, Present address: B.-S. Seo, Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer