caa a22 4500 475794915 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051465 doi (NATIONALLICENCE)springer-10.1007/s001220051465 Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa [Elektronische Daten] [N. Diwan, J. H. Bouton, G. Kochert, P. B. Cregan] Abstract : Cultivated alfalfa (Medicago sativa) is an autotetraploid. However, all three existing alfalfa genetic maps resulted from crosses of diploid alfalfa. The current study was undertaken to evaluate the use of Simple Sequence Repeat (SSR) DNA markers for mapping in diploid and tetraploid alfalfa. Ten SSR markers were incorporated into an existing F2 diploid alfalfa RFLP map and also mapped in an F2 tetraploid population. The tetraploid population had two to four alleles in each of the loci examined. The segregation of these alleles in the tetraploid mapping population generally was clear and easy to interpret. Because of the complexity of tetrasomic linkage analysis and a lack of computer software to accommodate it, linkage relationships at the tetraploid level were determined using a single-dose allele (SDA) analysis, where the presence or absence of each allele was scored independently of the other alleles at the same locus. The SDA diploid map was also constructed to compare mapping using SDA to the standard co-dominant method. Linkage groups were generally conserved among the tetraploid and the two diploid linkage maps, except for segments where severe segregation distortion was present. Segregation distortion, which was present in both tetraploid and diploid populations, probably resulted from inbreeding depression. The ease of analysis together with the abundance of SSR loci in the alfalfa genome indicated that SSR markers should be a useful tool for mapping tetraploid alfalfa. Springer-Verlag Berlin Heidelberg, 2000 Key words Medicago sativa nationallicence Autotetraploid linkage map nationallicence Microsatellites SSR nationallicence Inbreeding depression nationallicence Single dose allele (SDA) analysis nationallicence Diwan N. Soybean and Alfalfa Research Laboratory, USDA-ARS, Bldg. 006, Rm. 100, BARC-West, Beltsville, MD 20705, USA e-mail: peregan@asrr.arsusda.gov, Present address: N.D. Diwan, The Israeli Gene Bank for Agricultural Crops, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, US aut Bouton J. H. Department of Agronomy, University of Georgia, Athens, GA 30602, USA, US aut Kochert G. Department of Botany University of Georgia, Athens, GA 30602, USA, US aut Cregan P. B. Soybean and Alfalfa Research Laboratory, USDA-ARS, Bldg. 006, Rm. 100, BARC-West, Beltsville, MD 20705, USA e-mail: peregan@asrr.arsusda.gov, Present address: N.D. Diwan, The Israeli Gene Bank for Agricultural Crops, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, US aut https://doi.org/10.1007/s001220051465 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Diwan N. Soybean and Alfalfa Research Laboratory, USDA-ARS, Bldg. 006, Rm. 100, BARC-West, Beltsville, MD 20705, USA e-mail: peregan@asrr.arsusda.gov, Present address: N.D. Diwan, The Israeli Gene Bank for Agricultural Crops, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, US aut NATIONALLICENCE 700 1- Bouton J. H. Department of Agronomy, University of Georgia, Athens, GA 30602, USA, US aut NATIONALLICENCE 700 1- Kochert G. Department of Botany University of Georgia, Athens, GA 30602, USA, US aut NATIONALLICENCE 700 1- Cregan P. B. Soybean and Alfalfa Research Laboratory, USDA-ARS, Bldg. 006, Rm. 100, BARC-West, Beltsville, MD 20705, USA e-mail: peregan@asrr.arsusda.gov, Present address: N.D. Diwan, The Israeli Gene Bank for Agricultural Crops, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer