caa a22 4500 475794931 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051455 doi (NATIONALLICENCE)springer-10.1007/s001220051455 Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.) [Elektronische Daten] [E. Pestsova, E. Salina, A. Börner, V. Korzun, O. I. Maystrenko, M. S. Röder] Abstract : Ninety-five wheat microsatellite markers (WMS) were used to verify the authenticity of the set of Saratovskaya 29/Yanetzkis Probat inter-varietal wheat chromosome substitution lines developed using Saratovskaya 29 as the recipient variety. Polymorphic markers were available for all chromosome arms except 4DS, 6DS and 7DS. Each chromosome substitution line was tested by 2-8 microsatellite markers. The results demonstrate that most of the lines are correct. Out of 21 lines tested 17 showed the expected microsatellite pattern of the donor variety. Two entire chromosomes, 1B and 7A, and two chromosome arms, 3AL and 6DS, were not substituted with Yanetzkis Probat in their respective lines. Three microsatellite markers located in the distal regions of chromosome arms 4AL, 3BS and 5BL in the corresponding substitution lines did not reveal the expected microsatellite pattern of the recipient variety. The possible causes of the incorrect substitution line development and the appearance of incorrect distal microsatellite markers are discussed. The data confirm the idea that microsatellite markers provide ideal tools for testing the authenticity of genetic stocks of wheat. Springer-Verlag Berlin Heidelberg, 2000 Keywords Wheat nationallicence Chromosome substitutions nationallicence Microsatellite markers nationallicence Pestsova E. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut Salina E. Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia, RU aut Börner A. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut Maystrenko O. I. Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia, RU aut Röder M. S. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut https://doi.org/10.1007/s001220051455 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051455 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pestsova E. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut NATIONALLICENCE 700 1- Salina E. Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia, RU aut NATIONALLICENCE 700 1- Börner A. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut NATIONALLICENCE 700 1- Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut NATIONALLICENCE 700 1- Maystrenko O. I. Institute of Cytology and Genetics, Lavrentiev ave. 10, Novosibirsk, 630090, Russia, RU aut NATIONALLICENCE 700 1- Röder M. S. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany e-mail: pestsova@ipk-gatersleben.de, Fax: +49-39482-5137,, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer