caa a22 4500 47579494X CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051469 doi (NATIONALLICENCE)springer-10.1007/s001220051469 Assessment of Genetic Diversity in Three Subsets Constituted from the ICRISAT Sorghum Collection Using Random vs Non-Random Sampling ProceduresB. Using molecular markers [Elektronische Daten] B. Using molecular markers [C. Grenier, M. Deu, S. Kresovich, P. J. Bramel-Cox, P. Hamon] Abstract : The large size of the sorghum [Sorghum bi-color (L.) Moench] landrace collection maintained by ICRISAT lead to the establishment of a core collection. Thus, three subsets of around 200 accessions were established from: (1) a random sampling after stratification of the entire landrace collection (L), (2) a selective sampling based on quantitative characters (PCS), and (3) a selection based on the geographical origin of landraces and the traits under farmers' selection (T). An assessment was done of the genetic diversity retained by each sampling strategy using the polymorphisms at 15 microsatellite loci. The landraces of each subset were genotyped with three multiplex polymerase chain reactions (PCRs) of five fluorescent primer-pairs each with semi-automated allele sizing. The average allelic richness for each subset was equivalent (16.1, 16.3 and 15.4 alleles per locus for the subsets PCS, L, and T, respectively). The average genetic diversity was also comparable for the three subsets (0.81, 0.77 and 0.80 for the subsets PCS, L, and T, respectively). Allelic frequency distribution for each subset was compared with a chi-square test but few significant differences were observed. A high percentage of rare alleles (71 to 76% of 206 total rare alleles) was maintained in the three subsets. The global molecular diversity retained in each subset was not affected by a sampling procedure based upon phenotypic characters. Springer-Verlag Berlin Heidelberg, 2000 Key words Core collection nationallicence Sorghum nationallicence SSRs nationallicence Genetic diversity nationallicence Grenier C. CIRAD, TA 70/03, Avenue Agropolis, F-34398 Montpellier Cedex 5, France Fax: +33(0)467615509 e-mail: grenier@cirad.fr, FR aut Deu M. CIRAD, TA 70/03, Avenue Agropolis, F-34398 Montpellier Cedex 5, France Fax: +33(0)467615509 e-mail: grenier@cirad.fr, FR aut Kresovich S. Insitute for Genomic Diversity, Cornell University, Ithaca, NY 14853-2703, USA, US aut Bramel-Cox P. J. ICRISAT-GREP-GRD Patancheru, 502 324 A.P., India, IN aut Hamon P. Université Montpellier III/IRD, route de Mende, 34199 Montpellier Cedex 5, France, FR aut https://doi.org/10.1007/s001220051469 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051469 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grenier C. CIRAD, TA 70/03, Avenue Agropolis, F-34398 Montpellier Cedex 5, France Fax: +33(0)467615509 e-mail: grenier@cirad.fr, FR aut NATIONALLICENCE 700 1- Deu M. CIRAD, TA 70/03, Avenue Agropolis, F-34398 Montpellier Cedex 5, France Fax: +33(0)467615509 e-mail: grenier@cirad.fr, FR aut NATIONALLICENCE 700 1- Kresovich S. Insitute for Genomic Diversity, Cornell University, Ithaca, NY 14853-2703, USA, US aut NATIONALLICENCE 700 1- Bramel-Cox P. J. ICRISAT-GREP-GRD Patancheru, 502 324 A.P., India, IN aut NATIONALLICENCE 700 1- Hamon P. Université Montpellier III/IRD, route de Mende, 34199 Montpellier Cedex 5, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 475794818 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer