caa a22 4500 475794966 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001220051459 doi (NATIONALLICENCE)springer-10.1007/s001220051459 Variation in vigour and in RFLP-estimated heterozygosity by selfing tetraploid alfalfa: new perspectives for the use of selfing in alfalfa breeding [Elektronische Daten] [C. Scotti, F. Pupilli, S. Salvi, S. Arcioni] Abstract : The effect of selfing on vigour and heterozygosity was analysed in six independent families obtained by recurrent cycles of self-fertilization and selection until the S3 generation. The heterozygosity level, estimated by means of 11 homologous probes, decreased from the S0 to S3 generations and was partially restored in S2×S2 polycrosses. The decreasing trend was influenced by the inbreeding level of the chosen mother plant in each self generation to advance in subsequent selfing. Plant vigour estimated by Dry Matter Yield (DMY) decreased during inbreeding, and phenotypic selection of S2 individuals seemed to prove effective as the differences in DMY between vigorous and weak plants were maintained in S2×S2 crosses. No correlation between plant vigour and heterozygosity was found between subgroups of vigorous and weak plants selected within the same S2 family. Results are discussed with a view to selecting the best performing and least heterozygous plants during inbreeding to isolate useful genes and linkats in superior partially inbred parental lines. Springer-Verlag Berlin Heidelberg, 2000 Key words Alfalfa nationallicence Inbreeding depression nationallicence Heterozygosity nationallicence Plant vigour nationallicence Molecular markers nationallicence Scotti C. Istituto Sperimentale per le Colture Foraggere, V. le Piacenza 29, I-26900 Lodi, Italy, IT aut Pupilli F. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via della Madonna alta 130, I-06128 Perugia, Italy e-mail: S.Arcioni@irmgpf.pg.cnr.it, IT aut Salvi S. Istituto Sperimentale per le Colture Foraggere, V. le Piacenza 29, I-26900 Lodi, Italy, IT aut Arcioni S. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via della Madonna alta 130, I-06128 Perugia, Italy e-mail: S.Arcioni@irmgpf.pg.cnr.it, IT aut https://doi.org/10.1007/s001220051459 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051459 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scotti C. Istituto Sperimentale per le Colture Foraggere, V. le Piacenza 29, I-26900 Lodi, Italy, IT aut NATIONALLICENCE 700 1- Pupilli F. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via della Madonna alta 130, I-06128 Perugia, Italy e-mail: S.Arcioni@irmgpf.pg.cnr.it, IT aut NATIONALLICENCE 700 1- Salvi S. Istituto Sperimentale per le Colture Foraggere, V. le Piacenza 29, I-26900 Lodi, Italy, IT aut NATIONALLICENCE 700 1- Arcioni S. Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via della Madonna alta 130, I-06128 Perugia, Italy e-mail: S.Arcioni@irmgpf.pg.cnr.it, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer