caa a22 4500 475794982 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051605 doi (NATIONALLICENCE)springer-10.1007/s001220051605 The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars [Elektronische Daten] [C. D. Li, B. G. Rossnagel, G. J. Scoles] Abstract : Microsatellites have many desirable marker properties. There has been no report of the development and utilization of microsatellite markers in oat. The objectives of the present study were to construct oat microsatellite-enriched libraries, to isolate microsatellite sequences and evaluate their level of polymorphism in Avena species and oat cultivars. One hundred clones were isolated and sequenced from three oat microsatellite-libraries enriched for either (AC/TG) n , (AG/TC) n or (AAG/TTC) n repeats. Seventy eight clones contained microsatellites. A database search showed that 42% of the microsatellite flanking sequences shared significant homology with various repetitive elements. Alu and retrotransposon sequences were the two largest groups associated with the microsatellites. Forty four primer sets were used to amplify the DNA from 12 Avena species and 20 Avena sativa cultivars. Sixty two percent of the primers revealed polymorphism among the Avena species, but only 36% among the cultivars. In the cultivars, the microsatellites associated with repetitive elements were less polymorphic than those not associated with repetitive elements. Only 25% of the microsatellites associated with repetitive elements were polymorphic, while 46% of the microsatellites not associated with repetitive elements showed polymorphism in the cultivars. An average of four alleles with a polymorphism information content (PIC) of 0.57 per primer set was detected among the Avena species, and 3.8 alleles with a PIC of 0.55 among the cultivars. In addition, 54 barley microsatellite primers were tested in Avena species and 26% of the primers amplified microsatellites from oat. Using microsatellite polymorphisms, dendrograms were constructed showing phylogenetic relationships among Avena species and genetic relationships among oat cultivars. Springer-Verlag Berlin Heidelberg, 2000 Key words Enriched-library nationallicence SSR nationallicence Repetitive elements nationallicence Allelic diversity nationallicence Evolution nationallicence Li C. D. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut Rossnagel B. G. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut Scoles G. J. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut https://doi.org/10.1007/s001220051605 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051605 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li C. D. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut NATIONALLICENCE 700 1- Rossnagel B. G. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut NATIONALLICENCE 700 1- Scoles G. J. Department of Plant Sciences and Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada e-mail: graham.scoles@usask.ca Tel.: +1-306-966 5857, Fax: +1-306-966 5015, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer