caa a22 4500 475794990 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051597 doi (NATIONALLICENCE)springer-10.1007/s001220051597 Design of grapevine (Vitis vinifera L.) cultivar-specific SCAR primers for PCR fingerprinting [Elektronische Daten] [J. R. Vidal, P. Delavault, M. Coarer, A. Defontaine] Abstract : Among 34 grapevine cultivars (Vitis vinifera L.), eight putative genotype-specific RAPD markers, from 'Albariño', 'Caíño blanco', 'Chardonnay', 'Folle blanche', 'Grenache blanc', 'Malvasía Sitges', 'Torrontés' and 'Treixadura' respectively, were selected to transform into SCAR markers. Of these, seven markers were cloned and then five which showed a positive specific hybridization signal were sequenced. For these five markers, 30 sequence-specific primers ranging from 14 to 29 bases were designed to amplify genomic DNA from 64 grapevine cultivars under more-stringent PCR conditions. Only, two primer pairs, OpA111175p17R/ p17F and OpD10800p14R/p14F, still produced a specific SCAR marker, the 'Folle blanche' ScA111175 and the 'Malvasía Sitges' ScD10800 respectively. Moreover, the ScA111175 marker was amplified only in 'Folle blanche' among the 64 cultivars tested with a large annealing temperature range using either two different Taq DNA polymerases or two separate thermocyclers. In addition, we discuss the initial polymorphism originated by the RAPD technique and suggest a new design of SCAR primers to obtain reliable cultivar-specific SCAR markers from single PCR-based bands for identification purposes. Springer-Verlag Berlin Heidelberg, 2000 Key words 'Folle blanche' nationallicence Hybridization nationallicence RAPD nationallicence SCAR nationallicence Sequence-specific primer pair nationallicence Vitis vinifera L nationallicence Vidal J. R. Misión Biológica de Galicia. Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 28, E-36080 Pontevedra, Spain e-mail: jrv7@nysaes.cornell.edu Fax: +1-315-787 2216, Present address: Department of Horticultural Sciences, NYSAES, Cornell University, Geneva, NY 14456, USA, ES aut Delavault P. Groupe de Physiologie et Pathologie Végétales. Université de Nantes, 2 rue de la Houssinière, F-44322 Nantes, France, FR aut Coarer M. Centre Technique Interprofessionnel de la Vigne et du Vin (ITV France), Château de la Frémoire, F-44120 Vertou, France, FR aut Defontaine A. Laboratoire de Biotechnologie, UPRES Biocatalyse. Université de Nantes, 2 rue de la Houssinière, F-44322 Nantes, France, FR aut https://doi.org/10.1007/s001220051597 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051597 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vidal J. R. Misión Biológica de Galicia. Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 28, E-36080 Pontevedra, Spain e-mail: jrv7@nysaes.cornell.edu Fax: +1-315-787 2216, Present address: Department of Horticultural Sciences, NYSAES, Cornell University, Geneva, NY 14456, USA, ES aut NATIONALLICENCE 700 1- Delavault P. Groupe de Physiologie et Pathologie Végétales. Université de Nantes, 2 rue de la Houssinière, F-44322 Nantes, France, FR aut NATIONALLICENCE 700 1- Coarer M. Centre Technique Interprofessionnel de la Vigne et du Vin (ITV France), Château de la Frémoire, F-44120 Vertou, France, FR aut NATIONALLICENCE 700 1- Defontaine A. Laboratoire de Biotechnologie, UPRES Biocatalyse. Université de Nantes, 2 rue de la Houssinière, F-44322 Nantes, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer