caa a22 4500 475795008 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051609 doi (NATIONALLICENCE)springer-10.1007/s001220051609 Sequence variations at a complex microsatellite locus in rice and its conservation in cereals [Elektronische Daten] [A. P. Davierwala, W. Ramakrishna, P. K. Ranjekar, V. S. Gupta] Abstract : In an attempt to study changes associated with microsatellites in rice, the DNAs of cultivated rice, including indica and japonica varieties, and wild rice genotypes were amplified by the polymerase chain reaction with primers flanking the (GATA) n and (AC) n repeats at a microsatellite-containing locus OS1E6 (Genebank accession number AFO16647) previously reported from a PstI rice (var. Malkolam) genomic library in pUC18. Eight alleles of varying sizes were obtained which were cloned and sequenced. Sequencing data indicated that the size variations of the different alleles were due to differences in the repeat number as well as to sequence variations in the region flanking the microsatellite motifs. In order to study the presence of this complex microsatellite-containing locus of rice in different cereals, their DNAs were amplified using primers flanking the OS1E6 locus. It was found that this locus was present in the various cereal genotypes analyzed, indicating its conservation across different cereal members. Springer-Verlag Berlin Heidelberg, 2000 Key words Microsatellite nationallicence Sequence variation nationallicence Genetic diversity nationallicence Cereals nationallicence Davierwala A. P. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut Ramakrishna W. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut Ranjekar P. K. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut Gupta V. S. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut https://doi.org/10.1007/s001220051609 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051609 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davierwala A. P. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut NATIONALLICENCE 700 1- Ramakrishna W. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut NATIONALLICENCE 700 1- Ranjekar P. K. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut NATIONALLICENCE 700 1- Gupta V. S. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail: vidya@ems.ncl.res.in Tel.: 91-20 5893173/5893034, Fax: 91-20 5884032, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer