caa a22 4500 475795016 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051610 doi (NATIONALLICENCE)springer-10.1007/s001220051610 AFLP mapping of QTLs for in vitro organogenesis traits using recombinant inbred lines in sunflower (Helianthus annuus L.) [Elektronische Daten] [E. Flores Berrios, L. Gentzbittel, H. Kayyal, G. Alibert, A. Sarrafi] Abstract : Genetic control for two in vitro organogenesis traits, the number of shoots per explant plated (S/E) and the number of shoots per regenerating explant (S/RE), was investigated in 75 recombinant inbred lines (RILs) of sunflower and their two parents (PAC-2 and RHA-266). Genetic variability was observed among the 75 RILs for the organogenesis traits studied. Some RILs presented significant differences when compared with the best parental line (RHA-266). Genetic gain, in terms of the percentage of the best parent, for 32% of the selected RILs was significant. A set of 99RILs from the same cross including the 75 mentioned above was screened with 333 AFLP markers and a linkage map was constructed based on 264 linked loci. Six putative QTLs for the S/RE (tentatively named osr) and seven QTLs for the S/E (ose) trait were detected using composite interval mapping. For each trait, the QTLs explained 52% (ose) and 67% (osr) of the total phenotypic variance. These results suggested that additive gene effects predominate in explaining a large proportion of the observed genetic variation associated with regeneration ability. The coincidental location of QTLs for S/E and S/RE is discussed. Springer-Verlag Berlin Heidelberg, 2000 Keywords Sunflower nationallicence Helianthus annuus L nationallicence AFLP nationallicence Genetic mapping nationallicence in vitro regeneration nationallicence Recombinant inbred lines nationallicence Flores Berrios E. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut Gentzbittel L. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut Kayyal H. Department d'Amélioration des Plantes, Faculté d'Agriculture, Université de Damas, Syrie, XX aut Alibert G. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut Sarrafi A. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut https://doi.org/10.1007/s001220051610 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051610 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Flores Berrios E. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut NATIONALLICENCE 700 1- Gentzbittel L. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut NATIONALLICENCE 700 1- Kayyal H. Department d'Amélioration des Plantes, Faculté d'Agriculture, Université de Damas, Syrie, XX aut NATIONALLICENCE 700 1- Alibert G. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut NATIONALLICENCE 700 1- Sarrafi A. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18 Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail: sarrafi@ensat.fr Tel.: +33(0)5-62-19-35 80, Fax: +33(0)5-62-19-35 81, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer