caa a22 4500 475795040 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051594 doi (NATIONALLICENCE)springer-10.1007/s001220051594 Characterization of Aegilops uniaristata chromosomes by comparative DNA marker analysis and repetitive DNA sequence in situ hybridization [Elektronische Daten] [N. Iqbal, S. M. Reader, P. D. S. Caligari, T. E. Miller] Abstract : RFLP analyses were performed on wheat-Aegilops uniaristata Vis. addition and translocation lines to confirm the identity of added N-genome chromosomes. Complete 1N, 3N, 4N, 5N and 7N chromosome additions were identified, while the complete long arm and only part of the short arm was identified for chromosome 2N. There were no wheat-like 4/5 and 4/7 translocations in the Ae. uniaristata chromosomes. Chromosome 3N carried an asymmetric pericentric inversion, and the translocation line was a product of centric fusion between the long arms of chromosomes 3B and 3N. Chromosome-specific RAPD and microsatellite markers were also identified for all the added Ae. uniaristata chromosomes available in this set of addition lines. A new genomic in situ hybridization protocol combining pre-annealing of probe and blocking DNA and prehybridization with blocking DNA was developed to differentiate the very closely related genomes of Ae. uniaristata and wheat. Hybridization sites for the repetitive DNA sequences pAs1, pSc119.2 and pTa71 were identified on the N-genome chromosomes of Ae. uniaristata using the fluorescent in situ hybridization technique. Results showed deviation from the previously published ideogram of this species. A new ideogram, which shows the hybridization sites for the above sequences, was produced in which the chromosomes are arranged according to their homoeologous group. Springer-Verlag Berlin Heidelberg, 2000 Key words Addition lines nationallicence Aegilops uniaristata nationallicence In situ hybridization nationallicence Karyotype nationallicence Repetitive DNA sequences nationallicence RFLP analysis nationallicence Iqbal N. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut Reader S. M. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut Caligari P. D. S. Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut Miller T. E. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut https://doi.org/10.1007/s001220051594 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051594 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iqbal N. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut NATIONALLICENCE 700 1- Reader S. M. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut NATIONALLICENCE 700 1- Caligari P. D. S. Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut NATIONALLICENCE 700 1- Miller T. E. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK, Present address:N. Iqbal, Nuclear Institut for Agriculture and Biology, P.O. Box 128, Jang Road, Faisalbad, Pakistan. N. Iqbal, Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, Readng, RG6 6AS, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer