caa a22 4500 475795075 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051598 doi (NATIONALLICENCE)springer-10.1007/s001220051598 Development of STS markers closely linked to the Ppd-H1 photoperiod response gene of barley (Hordeum vulgare L.) [Elektronische Daten] [L. Decousset, S. Griffiths, R. P. Dunford, N. Pratchett, D. A. Laurie] Abstract : A BC2 population of 353 plants segregating for the Ppd-H1 photoperiod response gene was developed from a cross between the winter barley 'Igri' and the spring barley 'Triumph.' Bulk segregant analysis identified six AFLP markers closely linked to the Ppd-H1 gene and three strongly amplified AFLP bands that mapped 0.8-cM distal, 0.6-cM proximal and 2.3-cm proximal to Ppd-H1 were cloned and sequenced. Southern-blot analysis showed that the cloned fragments were single-copy sequences in 'Igri', the variety from which they were derived. Two of the sequences were absent from 'Triumph' while the third detected a single-copy sequence. The cloned fragments were used to design specific sequence tagged site (STS) primer pairs to assist in the construction of a high-resolution map of the Ppd-H1 region. Springer-Verlag Berlin Heidelberg, 2000 Keywords Amplified fragment length polymorphism (AFLP) nationallicence Barley (Hordeum vulgare) nationallicence Photoperiod nationallicence Mapping nationallicence Ppd-H1 nationallicence Sequence tagged site (STS) nationallicence Decousset L. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut Griffiths S. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut Dunford R. P. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut Pratchett N. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut Laurie D. A. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut https://doi.org/10.1007/s001220051598 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051598 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Decousset L. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut NATIONALLICENCE 700 1- Griffiths S. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut NATIONALLICENCE 700 1- Dunford R. P. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut NATIONALLICENCE 700 1- Pratchett N. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut NATIONALLICENCE 700 1- Laurie D. A. John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK e-mail: david.laurie@bbsrc.ac.uk Fax: +44 1603 450023 Present adress: L. Decousset BIOGEMMA, Site Universitaire des Cezeaux, 24 avenue des Landais, 63170 Aubiere, France, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer