caa a22 4500 475795083 CHVBK 20180406123721.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051599 doi (NATIONALLICENCE)springer-10.1007/s001220051599 Description and analysis of genetic diversity between commercial bean lines (Phaseolus vulgaris L.) [Elektronische Daten] [I. Métais, C. Aubry, B. Hamon, R. Jalouzot, D. Peltier] Abstract : The effectiveness of RFLP, DAMD-PCR, ISSR and RAPD markers in assessing polymorphism and relationships between 24 commercial lines of Phaseolus vulgaris L.was evaluated. We have used a Phaseolus-specific minisatellite sequence as a probe, which enabled 23 of the bean lines tested to be fingerprinted. Based on the sequence information obtained, primers corresponding to the bean-specific minisatellite core sequence were used in subsequent PCR amplifications. Our observations indicated that while the DAMD-PCR was sensitive in detecting genetic variation between bean species and between accessions of P. vulgaris, when used alone it may be limited in its ability to detect genetic variation among cultivated bean lines due to the low number of loci amplified. Only one out of the five ISSR primers tested was efficient in generating multiple band profiles, which was insufficient to distinguish all the different bean lines. Reproducible RAPD profiles were obtained, and these allowed us to differentiate all the genotypes tested with seven primers. We ultimately used only results from RFLP and RAPD markers to explore the genetic diversity among commercial bean lines. Both analyses led to the same clustering of the bean lines according to their geographical origins (United States or Europe). With respect to the European lines, the results obtained from RAPD data also enable the lines to be clustered according to their creators. Springer-Verlag Berlin Heidelberg, 2000 Key words Genetic diversity nationallicence Molecular markers nationallicence Common bean nationallicence Germplasm nationallicence Métais I. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut Aubry C. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut Hamon B. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut Jalouzot R. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut Peltier D. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut https://doi.org/10.1007/s001220051599 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051599 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Métais I. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut NATIONALLICENCE 700 1- Aubry C. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut NATIONALLICENCE 700 1- Hamon B. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut NATIONALLICENCE 700 1- Jalouzot R. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut NATIONALLICENCE 700 1- Peltier D. Laboratoire de Génétique, UFR Sciences, 2 bd Lavoisier, F-49045 Angers Cedex, France Fax: +33-0241735352, e-mail peltier@sciences.univ-angers.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer