caa a22 4500 475795113 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051606 doi (NATIONALLICENCE)springer-10.1007/s001220051606 Primers designed to amplify a mitochondrial nad1 intron in ponderosa pine, Pinus ponderosa, limber pine, P. flexilis, and Scots pine, P. sylvestris [Elektronische Daten] [J. B. Mitton, B. R. Kreiser, G. E. Rehfeldt] Abstract : The b/c intron of the mitochondrial nad1 gene, was sequenced to characterize the indel region of ponderosa pine, Pinus ponderosa. The sequence in ponderosa pine was aligned with the sequence in Scots pine, Pinus sylvestris, to design seven primers that are useful for sequencing and for revealing size variation in amplified fragments in ponderosa pine, Scots pine, and limber pine, Pinus flexilis. These primers reveal variability in all three species, and the pattern of variability within ponderosa pine is described by a preliminary survey. The indel region of ponderosa pine contains three distinct elements with lengths of 31, 32, and 34 bp. Springer-Verlag Berlin Heidelberg, 2000 Key words mtDNA nationallicence Polymorphism nationallicence Pinus ponderosa nationallicence Pinus flexilis nationallicence Pinus sylvestris nationallicence Mitton J. B. Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO, USA 80309, US aut Kreiser B. R. Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO, USA 80309, US aut Rehfeldt G. E. USDA Forest Service, Intermountain Research Station, 121 South Main Street, Moscow, ID, USA 83843, US aut https://doi.org/10.1007/s001220051606 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051606 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mitton J. B. Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO, USA 80309, US aut NATIONALLICENCE 700 1- Kreiser B. R. Department of Environmental, Population, and Organismic Biology, University of Colorado, Boulder, CO, USA 80309, US aut NATIONALLICENCE 700 1- Rehfeldt G. E. USDA Forest Service, Intermountain Research Station, 121 South Main Street, Moscow, ID, USA 83843, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer