caa a22 4500 475795121 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051608 doi (NATIONALLICENCE)springer-10.1007/s001220051608 Development, inheritance and cross-species amplification of microsatellite markers from Acacia mangium [Elektronische Daten] [P. A. Butcher, S. Decroocq, Y. Gray, G. F. Moran] Abstract : Microsatellite markers were developed in Acacia mangium Willd. to provide highly variable co-dominant markers for linkage mapping and studies of the breeding system. After an enrichment procedure 40% of colonies contained microsatellites in contrast with less than 1% from a non-enriched library. The majority of microsatellite sequences were AC repeats. Co-dominant segregation of alleles in two full-sib crosses of A. mangium was demonstrated at 33 microsatellite loci. The markers were highly variable relative to restriction fragment lengths polymorphisms (RFLPs). In the two pedigrees 53% of microsatellite loci were fully informative compared with 15% of RFLPs. Based on alleles detected among four parental genotypes, the microsatellites consisting of dinucleotide repeats were more polymorphic than those with tri- and tetra-nucleotide repeats. The microsatellite markers were not as transferable across species in the genus Acacia as RFLPs. Two thirds of the primers developed in A. mangium (subgenus Phyllodineae, section Juliflorae) amplified DNA from other species within the same section but failed to amplify in species from the subgenus Acacia. The availability of multiallelic, PCR-based, co-dominant microsatellite loci makes possible efficient studies of gene flow and breeding systems in A. mangium, a species with low allozyme variation. Springer-Verlag Berlin Heidelberg, 2000 Key words Acacia mangium nationallicence Microsatellites nationallicence Simple sequence repeats nationallicence Genome mapping nationallicence Butcher P. A. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut Decroocq S. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut Gray Y. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut Moran G. F. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut https://doi.org/10.1007/s001220051608 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051608 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Butcher P. A. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut NATIONALLICENCE 700 1- Decroocq S. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut NATIONALLICENCE 700 1- Gray Y. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut NATIONALLICENCE 700 1- Moran G. F. CSIRO Forestry and Forest Products, PO Box E4008, Kingston, ACT 2604, Australia e-mail: Penny.Butcher@ffp.csiro.au Tel.: +61-2-6281 8289, Fax. +61-2-6281 8233, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer