caa a22 4500 47579513X CHVBK 20180406123722.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s001220051611 doi (NATIONALLICENCE)springer-10.1007/s001220051611 Genotypic variation and chromosomal location of QTLs for somatic embryogenesis revealed by epidermal layers culture of recombinant inbred lines in the sunflower (Helianthus annuus L.) [Elektronische Daten] [E. Flores Berrios, A. Sarrafi, F. Fabre, G. Alibert, L. Gentzbittel] Abstract : The present study was conducted to identify the genetic factors controlling somatic embryogenesis in the sunflower. Two traits, the number of embryogenic explants per 40 explants plated (EE/40 E) and the number of embryos per 40 explants (E/40 E), were scored in 74 recombinant inbred lines (RILs) from a cross between 'PAC-2' and 'RHA-266'. The experiment was designed as a randomized complete block with 76 genotypes (74 recombinant inbred lines and two parents) and three replications. Each replication consisted of three Erlenmeyer flasks with 40 epidermal layers (explants). Analyses of variance indicated the existence of highly significant differences among parental genotypes and their RILs. Heritabilities for the somatic embryogenesis traits studied, EE/40 E and E/40 E, were high (0.64 and 0.77 respectively) and the genetic gain, in percentage of the best parent for 10% of selected RILs, was significant. Four QTLs for EE/40 E (tee) and seven for E/40 E (ete) were detected using composite interval mapping and AFLP mapping. The QTLs for EE/40 E explained 48% of the phenotypic variation while the QTLs for E/40 E explained about 89% of the variation. Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence Recombinant inbred lines nationallicence Somatic embryogenesis nationallicence Sunflower nationallicence QTL nationallicence Flores Berrios E. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut Sarrafi A. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut Fabre F. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut Alibert G. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut Gentzbittel L. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut https://doi.org/10.1007/s001220051611 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220051611 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Flores Berrios E. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut NATIONALLICENCE 700 1- Sarrafi A. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut NATIONALLICENCE 700 1- Fabre F. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut NATIONALLICENCE 700 1- Alibert G. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut NATIONALLICENCE 700 1- Gentzbittel L. Department of Biotechnology and Plant Breeding, BAP, INP-ENSAT 18, Chemin de Borde Rouge, BP 107, 31326 Castanet, France e-mail:sarrafi@ensat.fr Tel.: +33(0)5-62-19-3580, Fax: +33(0)5-62-19-3581, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer