caa a22 4500 475795156 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050072 doi (NATIONALLICENCE)springer-10.1007/s001220050072 Parental contribution and coefficient of coancestry among maize inbreds: pedigree, RFLP, and SSR data [Elektronische Daten] [R. Bernardo, J. Romero-Severson, J. Ziegle, J. Hauser, L. Joe, G. Hookstra, R. W. Doerge] Abstract : The genetic relationship between inbreds i and j can be estimated from pedigree or from molecular marker data. The objectives of this study were to: (1) determine whether pedigree, restriction fragment length polymorphism (RFLP), and simple sequence repeat (SSR) data give similar estimates of parental contribution and coefficient of coancestry (f ij ) among a set of maize (Zea mays L.) inbreds, and (2) compare the usefulness of RFLP and SSR markers for estimating genetic relationship. We studied 13 maize inbreds with known pedigrees. The inbreds were genotyped using 124 RFLP and 195 SSR markers. For each type of marker, parental contributions were estimated from marker similarity among an inbred and both of its parents, and were subsequently used to estimate f ij . Estimates of parental contribution differed significantly (α<0.05) between pedigree data and either type of marker, but not between the marker systems. The RFLP estimates of parental contribution failed to sum to 1.0, reflecting a higher frequency of non-parental bands with RFLP than with SSR markers. The f ij estimated from pedigree, RFLP, and SSR data were highly correlated (r=0.87-0.97), although significant differences were found among the three sets of f ij estimates. We concluded that pedigree and marker data often lead to different estimates of parental contribution and f ij , and that SSR markers are superior to RFLP markers for estimating genetic relationship. A relevant question is whether or not the inbreds previously genotyped with an older marker system (e.g., RFLP) need to be re-analyzed with a newer marker system (e.g., SSR) for the purpose of estimating genetic relationship. Such re-analysis seems unnecessary if data for the same type of marker are available for a given inbred and both of its parents. Springer-Verlag Berlin Heidelberg, 2000 Key words Genetic relationship nationallicence Maize nationallicence Pedigree nationallicence RFLP nationallicence SSR nationallicence Bernardo R. Department of Agronomy, Purdue University, 1150 Lilly Hall of Life Sciences, West Lafayette, IN 47907-1150, USA, US aut Romero-Severson J. Departments of Forestry and Natural Resources and Agronomy, Purdue University, 1159 Forestry Building, West Lafayette, IN 47907-1159, USA, US aut Ziegle J. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut Hauser J. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut Joe L. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut Hookstra G. PE AgGen, Inc., 1756 Picasso Avenue, Davis, CA 95616-0549, USA, US aut Doerge R. W. Departments of Statistics and Agronomy, Purdue University, 1399 Mathematical Sciences Building, West Lafayette, IN 47907-1399, USA, US aut https://doi.org/10.1007/s001220050072 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050072 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernardo R. Department of Agronomy, Purdue University, 1150 Lilly Hall of Life Sciences, West Lafayette, IN 47907-1150, USA, US aut NATIONALLICENCE 700 1- Romero-Severson J. Departments of Forestry and Natural Resources and Agronomy, Purdue University, 1159 Forestry Building, West Lafayette, IN 47907-1159, USA, US aut NATIONALLICENCE 700 1- Ziegle J. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut NATIONALLICENCE 700 1- Hauser J. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut NATIONALLICENCE 700 1- Joe L. PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA, US aut NATIONALLICENCE 700 1- Hookstra G. PE AgGen, Inc., 1756 Picasso Avenue, Davis, CA 95616-0549, USA, US aut NATIONALLICENCE 700 1- Doerge R. W. Departments of Statistics and Agronomy, Purdue University, 1399 Mathematical Sciences Building, West Lafayette, IN 47907-1399, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer