caa a22 4500 475795172 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050049 doi (NATIONALLICENCE)springer-10.1007/s001220050049 Structural differences in the vicinity of the waxy locus among the Oryza species with the AA-genome: identification of variable regions [Elektronische Daten] [H. Nagano, S. Kawasaki, Y. Kishima, Y. Sano] Abstract : We constructed a fine physical map for a 260-kb rice BAC contig surrounding the waxy locus. In order to identify variable regions within this 260-kb as to the restriction fragments length polymorphisms and copy numbers, sixty overlapping fragments derived from the 260-kb contig were used as probes to compare their corresponding structures among the Oryza species with AA-genome. According to the hybridization patterns, each fragment was classified into four types; true single copy (class 1), single copy with a smear background (class 2), multiple copy without a smear background (class 3), and only a smear background (class 4). Out of 16 single copy (class 1 and class 2) regions obtained in this map, the one site corresponding to wx gave rise to remarkable polymorphisms among AA-genome species in Oryza. In most of the fragments observed as repetitive segments (class 4), we could not find obvious differences in the hybridization pattern. However, interestingly, one site sorted into class-3 showed copy numbers varying among the lines. The lines belonging to O. sativa O. rufipogon, O. meridionalis,and O. longistaminata possessed high-copy numbers of this fragment, whereas only a few bands were detected in the lines from O. glaberrima, O. barthii, and O. glumaepatula. The two variable regions found within the AA-genome species represented genomic dynamisms. Springer-Verlag Berlin Heidelberg, 2000 Key words AA-genome nationallicence BAC clone nationallicence Oryza species nationallicence The waxy locus nationallicence Variable regions nationallicence Nagano H. Laboratory of Plant Breeding, Faculty of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan e-mail: rysano@abs.agr.hokudai.ac.jp Fax: +81-11-706-2439, JP aut Kawasaki S. National Institute of Agricultural Resources, Kan'non-dai 2-1-2, Ibaraki 305, Japan, JP aut Kishima Y. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan, JP aut Sano Y. Laboratory of Plant Breeding, Faculty of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan e-mail: rysano@abs.agr.hokudai.ac.jp Fax: +81-11-706-2439, JP aut https://doi.org/10.1007/s001220050049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nagano H. Laboratory of Plant Breeding, Faculty of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan e-mail: rysano@abs.agr.hokudai.ac.jp Fax: +81-11-706-2439, JP aut NATIONALLICENCE 700 1- Kawasaki S. National Institute of Agricultural Resources, Kan'non-dai 2-1-2, Ibaraki 305, Japan, JP aut NATIONALLICENCE 700 1- Kishima Y. Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan, JP aut NATIONALLICENCE 700 1- Sano Y. Laboratory of Plant Breeding, Faculty of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan e-mail: rysano@abs.agr.hokudai.ac.jp Fax: +81-11-706-2439, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer