caa a22 4500 475795210 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050048 doi (NATIONALLICENCE)springer-10.1007/s001220050048 RAPD and AFLP tagging and mapping of Beta (B) and Beta modifier (MoB), two genes which influence β-carotene accumulation in fruit of tomato (Lycopersicon esculentum Mill.) [Elektronische Daten] [Y. Zhang, J. R. Stommel] Abstract : The Beta (B) locus in tomato (Lycopersicon esculentum) increases fruit β-carotene content at the expense of lycopene, resulting in orange-pigmented fruit. Expression of B is influenced by the beta-modifier (Mo B ) gene which segregates independently of B. RAPD and AFLP analyses were performed using near isogenic lines (NILs) unique for B and bulked segregant analysis (BSA) of a L. esculentum×L. cheesmanii-derived F2 population segregating for B. Using 1018 random primers for RAPD analysis and 64 primer pairs for AFLP analysis, we identified polymorphic products which distinguished the NILs and the two bulked DNA samples constructed for BSA. A single 100 bp AFLP amplification product (E-ACA/M-CTG100) which distinguished the NILs cosegregated with Mo B and was demonstrated to be tightly linked to the locus. E-ACA/M-CTG100 exhibited a recombination frequency of 1.7% in the F2 progeny derived from an initial cross between the isolines. The Mo B locus was mapped to the long arm of chromosome 6. Two RAPD products (OPAR181100 and UBC792830) of 1100 bp and 830 bp, respectively, were polymorphic between orange- and red-fruited bulks constructed from F2 individuals in the L. esculentum and L. cheesmanii mating series. OPAR181100 and UBC792830 displayed recombination frequencies of 4.2% and 7.6%, respectively, in F2 progeny. The B-linked OPAR181100 marker was also mapped to the long arm of chromosome 6, proximal to Mo B , and revealed linkage between B and Mo B . Springer-Verlag Berlin Heidelberg, 2000 Key words Molecular marker nationallicence Bulk segregant analysis nationallicence Near-isogenic lines nationallicence Lycopersicon cheesmanii nationallicence Lycopersicon hirsutum nationallicence Zhang Y. US Department of Agriculture, Agricultural Research Service, Vegetable Laboratory, Plant Sciences Institute, Beltsville, MD 20705, USA e-mail: jstommel@asrr.arsusda.gov Fax: 301-504-5555, US aut Stommel J. R. US Department of Agriculture, Agricultural Research Service, Vegetable Laboratory, Plant Sciences Institute, Beltsville, MD 20705, USA e-mail: jstommel@asrr.arsusda.gov Fax: 301-504-5555, US aut https://doi.org/10.1007/s001220050048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Y. US Department of Agriculture, Agricultural Research Service, Vegetable Laboratory, Plant Sciences Institute, Beltsville, MD 20705, USA e-mail: jstommel@asrr.arsusda.gov Fax: 301-504-5555, US aut NATIONALLICENCE 700 1- Stommel J. R. US Department of Agriculture, Agricultural Research Service, Vegetable Laboratory, Plant Sciences Institute, Beltsville, MD 20705, USA e-mail: jstommel@asrr.arsusda.gov Fax: 301-504-5555, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer