caa a22 4500 475795237 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050082 doi (NATIONALLICENCE)springer-10.1007/s001220050082 Combining-ability analysis of somatic embryogenesis from epidermal layers in the sunflower (Helianthus annuus L.) [Elektronische Daten] [A. R. Bolandi, M. Branchard, G. Alibert, L. Genzbitel, A. Berville, A. Sarrafi] Abstract : Crosses were made between three cytoplasmic male-sterile and five restorer sunflower inbred lines. F1 hybrids, including their parents, were studied for their embryogenetic ability. Sterilized seeds were germinated in culture tubes on agar-solidified basal medium. Seven days after germination, epidermal layers from hypocotyls were transferred into MS and B5 liquid-midia for 5 and 8 days respectively. Then they were transferred to MS-120 solid-medium with a high level of sucrose (120 g/l). The experimental design was complete randomized blocks with three replications. Each replication per genotype consisted of three Erlenmeyer flasks with 20-25 epidermal layers (explants). Analysis of variance indicated the presence of significant variation among genotypes for all traits studied. General combining ability (GCA) and specific combining ability (SCA) showed significant effects for the studied traits. The highest value of GCA for the number of embryogenic explants per 100 explants plated (EE/100EP) belongs to parental female line 'CMS-PET1 B9'. This inberd line also gave a high positive GCA effect for the number of embryos per ten embryogenic explants (E/10EE). Additionally, it had the highest values for EE/100EP and E/10EE (41.70 and 19.28 respectively) and should be a promising parent in crossing programmes for the enhancement of somatic embryogenesis in the sunflower. The highest values of specific combining ability (SCA) for EE/100EP and E/10EE belong to the F1 hybrid 'CMS-PET1B9 'x' RT1B11' which has produced 53.45 embryogenic explants/100 explants and 9.67 embryos per ten embryogenic explants. Springer-Verlag Berlin Heidelberg, 2000 Key words Sunflower nationallicence Combining ability nationallicence Embryogenesis nationallicence Epidermal layer nationallicence Regeneration nationallicence Bolandi A. R. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut Branchard M. Laboratoire d'Amélioration des végétaux - Biotechnologie ISAMOR, Technopôle Brest-Iroise, F29280 Brest-Plouzane France, FR aut Alibert G. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut Genzbitel L. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut Berville A. INRA - Station de Génétique et Amélioration des Plantes - 2, place Pierre Viala, 34060 Montpellier, France, FR aut Sarrafi A. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut https://doi.org/10.1007/s001220050082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bolandi A. R. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Branchard M. Laboratoire d'Amélioration des végétaux - Biotechnologie ISAMOR, Technopôle Brest-Iroise, F29280 Brest-Plouzane France, FR aut NATIONALLICENCE 700 1- Alibert G. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Genzbitel L. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut NATIONALLICENCE 700 1- Berville A. INRA - Station de Génétique et Amélioration des Plantes - 2, place Pierre Viala, 34060 Montpellier, France, FR aut NATIONALLICENCE 700 1- Sarrafi A. Laboratoire de Biotechnologie et Amélioration des plantes, PBV, INP - ENSAT, UA 832 INRA, avenue de l'Agropiopôle, B.P. 107, F-31326 Castanet-Cedex France e-mail: sarrafi@ensat.fr, Fax : +33 (0) 5 62 19 35 81, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer