caa a22 4500 475795245 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050051 doi (NATIONALLICENCE)springer-10.1007/s001220050051 RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii) [Elektronische Daten] [T. Kondo, K. Terada, E. Hayashi, N. Kuramoto, M. Okamura, H. Kawasaki] Abstract : Linkage of RAPD markers to a single dominant gene for resistance to pine needle gall midge was investigated in Japanese black pine (Pinus thunbergii). Three primers that generated linked markers were found after 1160 primers were screened by bulked segregant analysis. The distances between the resistance gene, R, and the marker genes OPC06580, OPD01700, and OPAX192100 were 5.1 cM, 6.7 cM and 13.6 cM, respectively. OPC06580 was in coupling phase to R, whereas OPD01700 and OPAX192100 were in repulsion phase to R. A linkage map for a resistant tree was constructed using 96 macrogametophytes. In linkage analysis, 98 out of 127 polymorphic markers were assigned to 17 linkage groups and six linked pairs. The total length of this map was 1469.8 cM, with an average marker density of 15.6 cM. The genome length was estimated to be 2138.3 cM, and the derived linkage map covered 67.5% of the genome. Although the linked markers OPC06580, OPAX192100, and OPD01700, belonged to the same linkage group, no precise positions were found for OPC06580 or OPD01700. Springer-Verlag Berlin Heidelberg, 2000 Key words Pinus thunbergii nationallicence Pine needle gall midge nationallicence RAPD nationallicence Bulked segregant analysis nationallicence Linkage nationallicence Kondo T. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut Terada K. Tohoku Breeding Office, Forest Tree Breeding Center, Takizawa, Iwate 020-0173, Japan, JP aut Hayashi E. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut Kuramoto N. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut Okamura M. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut Kawasaki H. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut https://doi.org/10.1007/s001220050051 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050051 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kondo T. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut NATIONALLICENCE 700 1- Terada K. Tohoku Breeding Office, Forest Tree Breeding Center, Takizawa, Iwate 020-0173, Japan, JP aut NATIONALLICENCE 700 1- Hayashi E. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut NATIONALLICENCE 700 1- Kuramoto N. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut NATIONALLICENCE 700 1- Okamura M. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut NATIONALLICENCE 700 1- Kawasaki H. Forest Tree Breeding Center, Ishi, Juo, Taga, Ibaraki 319-1301, Japan e-mail: konkon@nftbc.affrc.go.jp, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer