caa a22 4500 475795253 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050064 doi (NATIONALLICENCE)springer-10.1007/s001220050064 Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance [Elektronische Daten] [A. Börner, S. Chebotar, V. Korzun] Abstract : The genetic identity of eight wheat (Triticum aestivum L.) accessions maintained in the Gatersleben genebank and regenerated up to 24 times was studied by using wheat microsatellite markers (WMS). It was demonstrated that WMS can be used to analyze bulks of seeds stored more than 50 years in a seed reference collection at room temperature. No contamination due to foreign pollen or incorrect handling during the multiplication cycles was discovered. For one accession (TRI 4599) genetic drift was observed, whereas for TRI 249 a heterogenous situation for two markers was maintained over the years. We were able to show that microsatellites can be used as a simple and reliable marker system for the verification of the integrity and genetic stability of genebank accessions. Springer-Verlag Berlin Heidelberg, 2000 Key words Ex-situ conservation nationallicence Genebank collection nationallicence Long term storage nationallicence Mikrosatellites nationallicence Wheat nationallicence Börner A. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Correnstrasse 3, D-06466 Gatersleben, Germany, DE aut Chebotar S. Plant Breeding and Genetics Institute, Ovidipolskaya dor. 3, 270036 Odessa, Ukraine, UA aut Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Correnstrasse 3, D-06466 Gatersleben, Germany, DE aut https://doi.org/10.1007/s001220050064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Börner A. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Correnstrasse 3, D-06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Chebotar S. Plant Breeding and Genetics Institute, Ovidipolskaya dor. 3, 270036 Odessa, Ukraine, UA aut NATIONALLICENCE 700 1- Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Correnstrasse 3, D-06466 Gatersleben, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer