caa a22 4500 475795261 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050059 doi (NATIONALLICENCE)springer-10.1007/s001220050059 Molecular phylogeny of mangroves VII. PCR-RFLP of trnS-psbC and rbcL gene regions in 24 mangrove and mangrove-associate species [Elektronische Daten] [M. Parani, M. Lakshmi, B. Ziegenhagen, M. Fladung, P. Senthilkumar, A. Parida] Abstract : Chloroplast DNA (cpDNA) regions, trnS-psbC and rbcL, from 120 individuals of 24 mangrove and mangrove associate species belonging to 11 orders, 13 families and 17 genera of Angiospermae were amplified by the polymerase chain reaction (PCR) and restriction-digested with HaeIII. Analysis of polymorphism in the restriction fragments (PCR-RFLP) revealed 18 classes of restriction banding pattern in trnS-psbC region. This has provided molecular evidence for diversity in the mangrove floral component at the above-species level. Intra-generic variations were observed in three genera, viz. Rhizophora, Avicennia and Suaeda. Species-specific restriction patterns were found in the genera Rhizophora and Suaeda. A natural hybrid belonging to the genus Rhizophora was also analysed, and its restriction pattern was the same as that of a putative parental species.PCR-RFLP analysis of rbcL gene region was less differentiating. However, it showed 13 different classes of restriction patterns and revealed the usefulness of these investigations for genome analysis at a higher taxonomic level. Intra-specific variation was not observed in any of the species in either of the cpDNA regions analysed. This is the first report which describes variations in the chloroplast genome of mangrove species. Springer-Verlag Berlin Heidelberg, 2000 Key words Mangroves nationallicence trnS-psbC nationallicence rbcL nationallicence cpDNA nationallicence PCR-RFLP nationallicence Phylogeny nationallicence Parani M. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut Lakshmi M. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut Ziegenhagen B. Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics, Sieker Landstraße 2, 22927 Großhansdorf, Germany, DE aut Fladung M. Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics, Sieker Landstraße 2, 22927 Großhansdorf, Germany, DE aut Senthilkumar P. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut Parida A. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut https://doi.org/10.1007/s001220050059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parani M. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut NATIONALLICENCE 700 1- Lakshmi M. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut NATIONALLICENCE 700 1- Ziegenhagen B. Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics, Sieker Landstraße 2, 22927 Großhansdorf, Germany, DE aut NATIONALLICENCE 700 1- Fladung M. Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics, Sieker Landstraße 2, 22927 Großhansdorf, Germany, DE aut NATIONALLICENCE 700 1- Senthilkumar P. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut NATIONALLICENCE 700 1- Parida A. M.S. Swaminathan Research Foundation, III Cross Street, Taramani Institution Area, Chennai 600 113, India e-mail: MDSAAA51@giasmd01.vsnl.net.in, Fax: +44-91-2351319, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer