caa a22 4500 47579527X CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050081 doi (NATIONALLICENCE)springer-10.1007/s001220050081 Host effect on genetic variation of Marssonina brunnea pathogenic to poplars [Elektronische Daten] [Z. M. Han, T. M. Yin, C. D. Li, M. R. Huang, R.L. Wu] Abstract : A broad collection was made for 42 isolates of Marssonina brunnea affecting poplar trees from three different sections (Leuce, Aigeiros and Tacamahaca) within the same Populus genus in China. Genetic diversity among these isolates was analyzed for morphological traits, cultural features, pathogenicity, hyphal anastomosis and randomly amplified polymorphic DNA markers (RAPDs). No significant difference was found in conidial morphological features, such as size, shape and septum location. Yet, considerable differences occur in other characteristics, which leads to the classification of the 42 isolates into two distinct groups, M. brunnea f.sp. monogermtubi and M. brunnea f.sp. multigermtubi. Isolates of M. brunnea f.sp. monogermtubi, derived from section Leuce, germinate only one germ tube, grow fast, produce dark-reddish conidiosorus clusters on the PDA medium, and are highly pathogenic to Populus tomentosa of section Leuce. By contrast, isolates of M. brunnea f.sp. multigermtubi, derived from sections Aigeiros and Tacamahaca, germinate 1-5 germ tubes, grow slowly, produce yellow-greenish conidiosorus clusters on PDA medium, and are pathogenic to Populus ×euramericana cv I-45 and Populus canadensis of section Aigeiros. DNA amplification using 11 RAPD primers generate 78 polymorphic bands among isolates. Cluster analyses based on RAPD markers broadly support such a classification by phenotypes, but provide a new insight into the possible origins of M. brunnea. It is proposed that the pathogen co-evolves with the poplars of section Leuce and has been subsequently distributed to the poplars of sections Aigeiros and Tacamahaca. An isolate from Populus adenopoda of section Leuce is placed in the third group, which is most likely a transmission type from M. brunnea f.sp. monogermtubi to M. brunnea f.sp. multigermtubi. Springer-Verlag Berlin Heidelberg, 2000 Key words Aigeiros nationallicence Leuce nationallicence Marssonina brunnea nationallicence Poplar nationallicence RAPD nationallicence Tacamahaca nationallicence Han Z. M. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut Yin T. M. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut Li C. D. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut Huang M. R. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut Wu R.L. Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203, USA e-mail: rwu@statgen.ncsu.edu Tel.: +1 (919)-515-1932, Fax: (919)-515-7315, US aut https://doi.org/10.1007/s001220050081 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050081 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Han Z. M. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut NATIONALLICENCE 700 1- Yin T. M. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut NATIONALLICENCE 700 1- Li C. D. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut NATIONALLICENCE 700 1- Huang M. R. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China, CN aut NATIONALLICENCE 700 1- Wu R.L. Program in Statistical Genetics, Department of Statistics, North Carolina State University, Raleigh, NC 27695-8203, USA e-mail: rwu@statgen.ncsu.edu Tel.: +1 (919)-515-1932, Fax: (919)-515-7315, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer