caa a22 4500 475795318 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050062 doi (NATIONALLICENCE)springer-10.1007/s001220050062 AFLP linkage group assignment to the chromosomes of Allium cepa L. via monosomic addition lines [Elektronische Daten] [A. W. van Heusden, M. Shigyo, Y. Tashiro, R. Vrielink-van Ginkel, C. Kik] Abstract : Two complete sets of Allium fistulosum L.- A. cepa monosomic addition lines (2n=2x+1=17) together with an AFLP linkage map based on a cross between A. cepa and A. roylei Stearn were used to re-evaluate the eight A. cepa linkage groups identified in the mapping study. The linkage groups could be assigned to individual, physical chromosomes. The low level of molecular homology between A. cepa and A. fistulosum enabled the identification of 186 amplified fragment length polymorphisms (AFLP™ markers) present in A. cepa and not in A. fistulosum with ten different primer combinations. With the monosomic addition lines the distribution of the markers over the eight chromosomes of A. cepa could be determined. Of these 186 AFLP markers 51 were absent in A. roylei and consequently used as markers in the mapping study (A. cepa ×A. roylei cross). Therefore, these 51 AFLP markers could be used to assign the eight A. cepa linkage groups identified in the mapping study to physical chromosomes. Seven isozyme and three CAPS markers were also included. Two of the linkage groups had to be split because they included two sets of markers corresponding to different chromosomes. A total of 20 (approx. 10%) of the A. cepa-specific AFLP markers were amplified in more than one type of the monosomic addition lines, suggesting unlinked duplications. The co-dominant isozyme and CAPS markers were used to identify the correspondence of linkage groupsoriginating from A. cepa or from A. roylei. Springer-Verlag Berlin Heidelberg, 2000 Key words Allium cepa nationallicence Monosomic addition lines nationallicence AFLP™ nationallicence CAPS nationallicence Isozymes nationallicence van Heusden A. W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut Shigyo M. Laboratory of Vegetable Crops, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan, JP aut Tashiro Y. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University, Saga 840-8502, Japan, JP aut Vrielink-van Ginkel R. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut https://doi.org/10.1007/s001220050062 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050062 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Heusden A. W. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut NATIONALLICENCE 700 1- Shigyo M. Laboratory of Vegetable Crops, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan, JP aut NATIONALLICENCE 700 1- Tashiro Y. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University, Saga 840-8502, Japan, JP aut NATIONALLICENCE 700 1- Vrielink-van Ginkel R. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut NATIONALLICENCE 700 1- Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, The Netherlands Fax: +31-317-418094 e-mail: a.w.vanheusden@cpro.dlo.nl, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer