caa a22 4500 475795334 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050075 doi (NATIONALLICENCE)springer-10.1007/s001220050075 Intraspecific genetic variability analysis of Neovossia indica causing Karnal bunt of wheat using repetitive elements [Elektronische Daten] [R. Datta, M. D. Rajebhosale, H. S. Dhaliwal, H-Singh,, P. K. Ranjekar, V. S. Gupta] Abstract : Neovossia indica (Tilletia indica), causing Karnal bunt of wheat, affects major wheat growing regions all over the world. Karnal bunt ranks as one of the major diseases of wheat causing quality losses and monetary losses due to international quarantine regulations. The present work is the first report of a genetic diversity analysis of Indian isolates of N. indica. A library of N. indica isolate Ni7 was constructed in a λZAPII system, and three repetitive elements were identified for molecular analysis. These repetitive elements generated complex hybridization profiles producing fingerprint patterns of all seven isolates. Copy-number estimation of these three elements, pNiR9, pNiR12 and pNiR16, indicated the presence of 32, 61 and 64 copies, respectively. Cluster analysis based on hybridization patterns grouped together moderately virulent isolates Ni1, Ni7 and Ni8, thus suggesting a positive correlation between virulence typing and cluster analysis based on molecular data. Variability analysis of N. indica isolates will aid in checking new resistant sources in host germplasm. Springer-Verlag Berlin Heidelberg, 2000 Key words Karnal bunt nationallicence Repetitive elements nationallicence Genome diversity nationallicence RFLP nationallicence Datta R. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut Rajebhosale M. D. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut Dhaliwal H. S. Biotechnology Centre, Punjab Agricultural University, Ludhiana 141004, India, IN aut H-Singh, Biotechnology Centre, Punjab Agricultural University, Ludhiana 141004, India, IN aut Ranjekar P. K. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut Gupta V. S. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut https://doi.org/10.1007/s001220050075 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050075 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Datta R. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut NATIONALLICENCE 700 1- Rajebhosale M. D. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut NATIONALLICENCE 700 1- Dhaliwal H. S. Biotechnology Centre, Punjab Agricultural University, Ludhiana 141004, India, IN aut NATIONALLICENCE 700 1- H-Singh Biotechnology Centre, Punjab Agricultural University, Ludhiana 141004, India, IN aut NATIONALLICENCE 700 1- Ranjekar P. K. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut NATIONALLICENCE 700 1- Gupta V. S. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India e-mail:Vidya@ems.ncl.res.in Fax: +91-020-5884032, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer