caa a22 4500 475795407 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050056 doi (NATIONALLICENCE)springer-10.1007/s001220050056 AFLP-derived STS markers for the identification of sex in Asparagus officinalis L [Elektronische Daten] [S. M. Reamon-Büttner, C. Jung] Abstract : For a simple, rapid and PCR-based screening of sex in the cultivated asparagus (Asparagus officinalis L.), we developed five STS markers from previously mapped, low-copy, sex-linked AFLP markers. A male/female PCR assay was feasible with these STS markers either by direct amplification or by digestion with restriction enzymes. Similar to the AFLP markers from which they were derived, STS4150.1, STS4150.2, STS4150.3 and STS3156 did not give recombinants in five different populations. STS3660 could be scored codominantly, enabling the differentiation of XY from YY males in the screened F2 mapping population. The use of the sex-linked STS markers should allow early identification of sex, thus accelerating the breeding process for new asparagus varieties. Further, 10 additional AFLP markers obtained with PstI/MseI primer combinations have been mapped on the L5 chromosome, bringing the total number of known AFLP and STS markers flanking the sex locus to 24. These markers can be utilized for fine mapping of the sex gene in asparagus, which will pave the way for a map-based cloning approach. Springer-Verlag Berlin Heidelberg, 2000 Key words AFLP nationallicence STS nationallicence Sex-linked DNA markers nationallicence PCR markers nationallicence Asparagus officinalis L nationallicence Reamon-Büttner S. M. Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Fax: +49 (431) 880 2566, DE aut Jung C. Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Fax: +49 (431) 880 2566, DE aut https://doi.org/10.1007/s001220050056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reamon-Büttner S. M. Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Fax: +49 (431) 880 2566, DE aut NATIONALLICENCE 700 1- Jung C. Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Fax: +49 (431) 880 2566, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer