caa a22 4500 475795431 CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050083 doi (NATIONALLICENCE)springer-10.1007/s001220050083 Development of SCAR markers linked to the gene Or5 conferring resistance to broomrape (Orobanche cumana Wallr.) in sunflower [Elektronische Daten] [Y. H. Lu, J. M. Melero-Vara, J. A. García-Tejada, P. Blanchard] Abstract : A consensus molecular linkage map of 61.9 cM containing the Or5 gene, which confers resistance to race E of broomrape orobanche cumana, five SCAR markers (three dominant, two codominant) and one RAPD marker were identified based on segregation data scored from two F2 populations of susceptible×resistant sunflower line crosses. Bulked segregant analysis was carried out to generate the five SCAR markers, while the single RAPD marker in the group was identified from 61 segregating RAPD markers that were directly screened on one of the two F2 populations. The five SCAR markers, RTS05, RTS28, RTS40, RTS29 and RTS41, were significantly (LOD≥4.0) linked to the Or5 gene and mapped separately at 5.6, 13.6, 14.1, 21.4 and 39.4 cM from the Or5 locus on one side, while the RAPD marker, UBC120_660, was found at 22.5 cM (LOD=1.4) on the opposite side. These markers should facilitate the efficient transfer of the resistance gene among sunflower breeding lines. As the first report on molecular markers linked to a broomrape resistance gene, the present work provides a starting point to study other genes and to examine the hypothesis of the clustering of broomrape resistance genes in sunflower. Springer-Verlag Berlin Heidelberg, 2000 Key words SCAR nationallicence RAPD nationallicence Bulked segregant analysis nationallicence Marker-assisted selection nationallicence Orobanche cumana nationallicence Helianthus annuus nationallicence Lu Y. H. Rustica Prograin Génétique, Unité marquage génétique, 7 rue Hermès, 31520 Ramonville Saint-Agne, France e-mail: phblanch@rustica-prograin.fr, FR aut Melero-Vara J. M. Instituto de Agricultura Sostenible, C.S.I.C., Apdo. 4084, 14080 Córdoba, Spain, ES aut García-Tejada J. A. Arlesa Semillas S.A., Apdo. 1239, 41080 Sevilla, Spain, ES aut Blanchard P. Rustica Prograin Génétique, Unité marquage génétique, 7 rue Hermès, 31520 Ramonville Saint-Agne, France e-mail: phblanch@rustica-prograin.fr, FR aut https://doi.org/10.1007/s001220050083 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050083 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Y. H. Rustica Prograin Génétique, Unité marquage génétique, 7 rue Hermès, 31520 Ramonville Saint-Agne, France e-mail: phblanch@rustica-prograin.fr, FR aut NATIONALLICENCE 700 1- Melero-Vara J. M. Instituto de Agricultura Sostenible, C.S.I.C., Apdo. 4084, 14080 Córdoba, Spain, ES aut NATIONALLICENCE 700 1- García-Tejada J. A. Arlesa Semillas S.A., Apdo. 1239, 41080 Sevilla, Spain, ES aut NATIONALLICENCE 700 1- Blanchard P. Rustica Prograin Génétique, Unité marquage génétique, 7 rue Hermès, 31520 Ramonville Saint-Agne, France e-mail: phblanch@rustica-prograin.fr, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer