caa a22 4500 47579544X CHVBK 20180406123722.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050060 doi (NATIONALLICENCE)springer-10.1007/s001220050060 Evidence of multiple complex patterns of T-DNA integration into the rice genome [Elektronische Daten] [Z. Yin, G.-L. Wang] Abstract : The transfer of the long T-DNA (T-DNA and non-T-DNA) of a binary plasmid from Agrobacterium into the rice genome was investigated at both molecular and genetic levels. Out of 226 independent transgenic plants, 33% of the transformants contained non-T-DNA sequences. There was no major difference in the frequency of non-T-DNA transfer among three Agrobacterium tumefaciens strains.Four T1 plants containing a single putative long T-DNA insertion were selected for Southern analysis. Three of them were confirmed to have a long T-DNA insertion with a size of greater-than-unit-length of the binary plasmid. This was further confirmed by rescuing the intact binary plasmid from these plants. Our results suggest that long T-DNA transfer by rolling-circle replication from Agrobacterium to rice occurs frequently, and that the high frequency of non-T-DNA transfer should be considered when producing transgenic rice for commercial production. Springer-Verlag Berlin Heidelberg, 2000 Key words Agrobacterium tumefaciens nationallicence T-DNA nationallicence Non-T-DNA nationallicence Long T-DNA transfer nationallicence Rice transformation nationallicence Yin Z. Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Republic of Singapore e-mail: wanggl@ima.org.sg Fax: 65-872 7007, SG aut Wang G.-L Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Republic of Singapore e-mail: wanggl@ima.org.sg Fax: 65-872 7007, SG aut https://doi.org/10.1007/s001220050060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yin Z. Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Republic of Singapore e-mail: wanggl@ima.org.sg Fax: 65-872 7007, SG aut NATIONALLICENCE 700 1- Wang G.-L Institute of Molecular Agrobiology, 1 Research Link, The National University of Singapore, Singapore 117604, Republic of Singapore e-mail: wanggl@ima.org.sg Fax: 65-872 7007, SG aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer