caa a22 4500 475795482 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050053 doi (NATIONALLICENCE)springer-10.1007/s001220050053 A novel hypersensitive resistance response against potato virus A in cultivar 'Shepody' [Elektronische Daten] [R. P. Singh, X. Nie, G. C. C. Tai] Abstract : The potato cultivar 'Shepody' is susceptible to a number of potato viruses including potato virus Y (PVY, potyvirus) but was found to possess extreme resistance to another potyvirus, potato virus A (PVA). 'Shepody' plants were resistant to PVA infection in manual and graft inoculations. PVA replication was not detected in any of the inoculated plants by ELISA, an infectivity assay and RT-PCR. However, 'Shepody' plants grafted with shoots containing PVA developed a novel symptomology which resembled a virus infection in appearance and in rate of translocation to the entire plant. Efforts to transmit the symptom-inducing agent manually failed. Graft-inoculation to potato virus indicator plants and PVA-susceptible potato plants showed that the symptom inducer was PVA at an extremely low concentration, detected using RT-PCR followed by Southern blot assay. Tubers from grafted but resistant 'Shepody' plants had necrotic surfaces and internal spots. PVA was detected from necrotic areas but not from the non-necrotic ones. However, plants resulting from necrotic tubers were free from aerial leaf symptoms observed in grafted plants and produced non-necrotic normal tubers. A trace-back of the parental lineage of 'Shepody' indicated that the resistance had been introgressed from the cultivar 'Bake King'. Analysis of progeny of a cross of resistant 'Shepody' to the susceptible 'Goldrus' indicated that this resistance is controlled by two independent dominant complementary genes in contrast to monogenic resistance reported for other potato viruses. Springer-Verlag Berlin Heidelberg, 2000 Key words Complementary genes nationallicence Extreme virus resistance nationallicence Genetics nationallicence Necrotic tubers nationallicence Restricted virus distribution nationallicence Solanum tuberosum nationallicence Singh R. P. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut Nie X. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut Tai G. C. C. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut https://doi.org/10.1007/s001220050053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh R. P. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut NATIONALLICENCE 700 1- Nie X. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut NATIONALLICENCE 700 1- Tai G. C. C. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7 Fax +1506.452-3316 e-mail: singhr@em.agr.ca, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer