caa a22 4500 475795539 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050076 doi (NATIONALLICENCE)springer-10.1007/s001220050076 Genetic linkage mapping in Acacia mangium 1. Evaluation of restriction endonucleases, inheritance of RFLP loci and their conservation across species [Elektronische Daten] [P. A. Butcher, G. F. Moran, R. Bell] Abstract : Random genomic probes were used to assess levels of restriction fragment length polymorphism (RFLP) in two 2-generation outbred pedigrees of Acacia mangium Willd. Probes were evaluated for their ability to detect polymorphic loci in each pedigree and to determine the relative efficiency of different restriction enzymes in revealing polymorphisms. Sixty two percent of the probes which detected single- or low-copy number sequences revealed polymorphisms with at least one restriction enyzme. HpaII was the most efficient in detecting polymorphism among first-generation individuals. The recognition sequence of HpaII contains a CpG dimer, suggesting that cytosines in the CpG sequence may be hotspots for mutation in plant genomes, as previously reported in bacterial and mammalian genomes. Mendelian inheritance of 230 loci was demonstrated based on single-locus segregation in second-generation individuals. Less than 5% of loci showed evidence of segregation distortion. The proportion of fully informative loci (15%) was lower than previously reported in eucalypts reflecting the lower level of genetic diversity in A. mangium. The RFLP probes are suitable for the construction of a high-density genetic linkage map in A. mangium. Cross-hybridisation of the A.mangium RFLPs to DNA from species representing the three subgenera of the genus Acacia indicates that these markers could be used in breeding programs of other diploid acacias, for comparative studies of genome organisation, and for phylogenetic studies. Springer-Verlag Berlin Heidelberg, 2000 Key words Acacia nationallicence RFLP nationallicence Restriction enzymes nationallicence Genome mapping nationallicence Butcher P. A. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut Moran G. F. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut Bell R. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut https://doi.org/10.1007/s001220050076 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050076 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Butcher P. A. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut NATIONALLICENCE 700 1- Moran G. F. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut NATIONALLICENCE 700 1- Bell R. CSIRO Forestry and Forest Products, PO Box E4008, Kingston ACT 2604, Australia e-mail: Penny.Butcher @ffp.csiro.au, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer