caa a22 4500 475795547 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050074 doi (NATIONALLICENCE)springer-10.1007/s001220050074 Genetic mapping of the powdery mildew resistance gene Pm6 in wheat by RFLP analysis [Elektronische Daten] [W. Tao, D. Liu, J. Liu, Y. Feng, P. Chen] Abstract : Pm6 in bread wheat (Triticum aestivum L.), which was transferred from Triticum. timopheevii L., is a gene conferring resistance to the powdery mildew disease caused by Erysiphe graminis f. sp. tritici. Six near-isogenic lines ( NILs ) of Pm6 in a cultivar 'Prins' background were analyzed to map this gene using restriction fragment length polymorphism (RFLP). Each of the six NILs possessed a T. timopheevii-derived segment, varying in length, and associated with powdery mildew resistance. Lines IGV1-465 (FAO163b/ 7*Prins) and IGV1-467 (Idaed 59B/7*Prins) had the shortest introgressed segments, which were detected only by DNA probes BCD135 and PSR934, respectively. The polymorphic loci detected by both probes were mapped to the long arm of chromosome 2B. Lines IGV1-458 (CI13250/7*Prins) and IGV1-456 (CI12559/8*Prins) contained the longest T. timopheevii segments involving both arms of donor chromosome 2G across the centromere. All these introgressed segments had an overlapping region flanked by the loci xpsr934 and xbcd135 on 2BL. Thus, Pm6 was located in this region since the powdery mildew resistance in all the NILs resulted from the introgressed fragments. Using the F2 mapping population from a cross of IGV1-463 (PI170914/7*Prins)×Prins, Pm6 was shown to be closely linked to the loci xbcd135 and xbcd266 at a genetic distance of 1.6 cM and 4.8 cM, respectively. BCD135 was successfully used in detecting the presence of Pm6 in different genetic backgrounds. Springer-Verlag Berlin Heidelberg, 2000 Key words Triticum aestivum nationallicence Tritiam timopheevii nationallicence Pm6 nationallicence Introgression lines nationallicence RFLP nationallicence Tao W. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut Liu D. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut Liu J. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut Feng Y. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut Chen P. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut https://doi.org/10.1007/s001220050074 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050074 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tao W. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut NATIONALLICENCE 700 1- Liu D. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut NATIONALLICENCE 700 1- Liu J. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut NATIONALLICENCE 700 1- Feng Y. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut NATIONALLICENCE 700 1- Chen P. Key Open Laboratory of Agriculture Ministry for Crop Cytogenetics, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China e-mail: djliu@public1.ptt.js.cn, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer