caa a22 4500 475795555 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001220050066 doi (NATIONALLICENCE)springer-10.1007/s001220050066 Hot spots of DNA instability revealed through the study of somaclonal variation in rye [Elektronische Daten] [R. Linacero, E. Freitas Alves, A. M. Vázquez] Abstract : RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences. From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one plant or in several but originated from the same callus (variable band). In other cases (5.25%) the same band varied in several plants obtained from different calli. We call these hypervariable bands and they could vary between plants belonging to different cultivars and/or with different origins, inflorescences or embryos. Thus, they must originate through independent mutational events. We assume that these bands represent hypervariable regions of the rye genome and so detect hot spots of DNA instability. Some of these bands proved to be unique sequences, others were present in a low copy number while the remaining ones were moderately or highly repetitive. Springer-Verlag Berlin Heidelberg, 2000 Key words Rye nationallicence DNA instability nationallicence Hypervariable sequences nationallicence Somaclonal variation nationallicence RAPD nationallicence Linacero R. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut Freitas Alves E. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut Vázquez A. M. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut https://doi.org/10.1007/s001220050066 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050066 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linacero R. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut NATIONALLICENCE 700 1- Freitas Alves E. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut NATIONALLICENCE 700 1- Vázquez A. M. Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer