caa a22 4500 47579558X CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00002903 doi (NATIONALLICENCE)springer-10.1007/PL00002903 The use of self-pollinated progenies as 'in-groups' for the genetic characterization of cocoa germplasm [Elektronische Daten] [Y. M. Charters, M. J. Wilkinson] Theobroma cacao: ) genotypes. Six primers were sufficient to distinguish all but three pairs of the 62 accessions examined. A UPGMA dendrogram was used to provide a measure of the genetic variability between genotypes. The scale was supplied by the inclusion of Theobroma grandiflora as an 'out group' and also by the use of two contrasting progenies as 'in groups'. The 'in groups' were obtained from the self-pollination of one plant (SPEC 54.1) known to be highly homozygousand also of a second, highly heterozygous, clone (P 19B). These reference points allowed several documentation errors to be resolved and provided a basis for identifying unwanted or low-priority material. Implications of the work for the routine maintenance of large germplasm collections are briefly discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Theobroma cacao nationallicence ISSR PCR nationallicence Microsatellites nationallicence Germplasm nationallicence Genebank maintenance nationallicence Charters Y. M. Department of Agricultural Botany, School of Plant Sciences, Whiteknights, P.O. Box 221, The University of Reading, RG6 6AS, UK, GB aut Wilkinson M. J. Department of Agricultural Botany, School of Plant Sciences, Whiteknights, P.O. Box 221, The University of Reading, RG6 6AS, UK, GB aut https://doi.org/10.1007/PL00002903 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00002903 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Charters Y. M. Department of Agricultural Botany, School of Plant Sciences, Whiteknights, P.O. Box 221, The University of Reading, RG6 6AS, UK, GB aut NATIONALLICENCE 700 1- Wilkinson M. J. Department of Agricultural Botany, School of Plant Sciences, Whiteknights, P.O. Box 221, The University of Reading, RG6 6AS, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer