caa a22 4500 47579561X CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050016 doi (NATIONALLICENCE)springer-10.1007/s001220050016 Construction of a yeast artificial chromosome library of pepper ( Capsicum annuum L.) and identification of clones from the Bs2 resistance locus [Elektronische Daten] [T. Tai, B. J. Staskawicz] Abstract : A yeast artificial chromosome (YAC) library was constructed using high-molecular-weight DNA isolated from pepper (Capsicum annuum L.) leaf protoplasts. Insert DNA was prepared by partial digestion using EcoRI and subjected to electrophoretic fractionation before in-gel ligation to the pJS97/98 YAC vector. Prior to transformation of yeast spheroplasts, ligation products were subjected to a second electrophoretic size selection. The library consists of about 19 000 clones with an average insert size of 500 kb, thus representing approximately three haploid genome equivalents. Three PCR-based markers tightly linked to the pepper Bs2 resistance gene were used to assess the utility of this library for positional cloning. Three YAC clones containing pepper genomic DNA from the Bs2 resistance locus were isolated from the library. The clones ranged in size from 270 kb to 1.2 Mb and should prove useful for the cloning of the Bs2 gene. Springer-Verlag Berlin Heidelberg, 2000 Key words Pepper nationallicence YAC library nationallicence Positional cloning nationallicence Resistance gene nationallicence Bs2 nationallicence Tai T. Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA e-mail: stask@nature.berkeley.edu, Fax: 510-6937955, US aut Staskawicz B. J. Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA e-mail: stask@nature.berkeley.edu, Fax: 510-6937955, US aut https://doi.org/10.1007/s001220050016 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050016 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tai T. Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA e-mail: stask@nature.berkeley.edu, Fax: 510-6937955, US aut NATIONALLICENCE 700 1- Staskawicz B. J. Department of Plant Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA e-mail: stask@nature.berkeley.edu, Fax: 510-6937955, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer