caa a22 4500 475795636 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050003 doi (NATIONALLICENCE)springer-10.1007/s001220050003 Introgression of Allium fistulosum into A. cepa mediated by A. roylei [Elektronische Daten] [L. I. Khrustaleva, C. Kik] Abstract : Introgression of Allium fistulosum into the genome of A. cepa using A. roylei as a bridging species was studied by means of genomic in situ hybridization (GISH). Here we demonstrate for the first time that A. fistulosum can be stably introgressed into A. cepa with a bridge-cross. The first and second bridge-cross generations were fertile, although pollen was sterile in some individuals. Only occasionally were there translocations in the second generation bridge-cross. Recombination between the three genomes was frequently seen in meiotic anaphase 1 and prophase 2 chromosomes of the first generation bridge cross and in mitotic chromosomes of the second generation bridge-cross. The number of observed recombination points in anaphase 1 and prophase 2 significantly exceeded the value expected from chiasma frequency in metaphase 1. Recombination points were randomly distributed, thus the A. cepa or A. roylei type of random distribution prevails over the A. fistulosum type of proximally localised chiasmata. Springer-Verlag Berlin Heidelberg, 2000 Key words Onion nationallicence Wild species nationallicence Multi-colour genomic in situ hybridisation nationallicence Chiasmata nationallicence Meiotic recombination nationallicence Khrustaleva L. I. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: c.kik@cpro.dlo.nl, Fax: 31 317 418094, NL aut Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: c.kik@cpro.dlo.nl, Fax: 31 317 418094, NL aut https://doi.org/10.1007/s001220050003 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khrustaleva L. I. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: c.kik@cpro.dlo.nl, Fax: 31 317 418094, NL aut NATIONALLICENCE 700 1- Kik C. DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Vegetable and Fruit Crops, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands e-mail: c.kik@cpro.dlo.nl, Fax: 31 317 418094, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer