caa a22 4500 475795687 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00002904 doi (NATIONALLICENCE)springer-10.1007/PL00002904 Locating introgressions of Hordeum bulbosum chromatin within the H. vulgare genome [Elektronische Daten] [R. A. Pickering, S. Malyshev, G. Künzel, P. A. Johnston, V. Korzun, M. Menke, I. Schubert] Abstract : Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL. Springer-Verlag Berlin Heidelberg, 2000 Key words Hordeum vulgare nationallicence Hordeum bulbosum nationallicence Leaf rust resistance nationallicence Gene introgression nationallicence In situ hybridization nationallicence Pickering R. A. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut Malyshev S. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut Künzel G. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut Johnston P. A. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut Menke M. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut Schubert I. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut https://doi.org/10.1007/PL00002904 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00002904 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pickering R. A. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut NATIONALLICENCE 700 1- Malyshev S. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Künzel G. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Johnston P. A. New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand, NZ aut NATIONALLICENCE 700 1- Korzun V. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Menke M. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut NATIONALLICENCE 700 1- Schubert I. Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer