caa a22 4500 475795695 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050019 doi (NATIONALLICENCE)springer-10.1007/s001220050019 Molecular analysis of introgressive breeding in coffee ( Coffea arabica L.) [Elektronische Daten] [P. Lashermes, S. Andrzejewski, B. Bertrand, M. C. Combes, S. Dussert, G. Graziosi, P. Trouslot, F. Anthony] Abstract : Nineteen arabica coffee introgression lines (BC1F4) and two accessions derived from a spontaneous interspecific cross (i.e. Timor Hybrid) between Coffea arabica (2n=4x=44) and C. canephora (2n=2x=22) were analysed for the introgression of C. canephora genetic material. The Timor Hybrid-derived genotypes were evaluated by AFLP, using 42 different primer combinations, and compared to 23 accessions of C. arabica and 8 accessions of C. canephora. A total of 1062 polymorphic fragments were scored among the 52 accessions analysed. One hundred and seventy-eight markers consisting of 109 additional bands (i.e. introgressed markers) and 69 missing bands distinguished the group composed of the Timor Hybrid-derived genotypes from the accessions of C. arabica. AFLP therefore seemed to be an extremely efficient technique for DNA marker generation in coffee as well as for the detection of introgression in C. arabica. The genetic diversity observed in the Timor Hybrid-derived genotypes appeared to be approximately double that in C. arabica. Although representing only a small proportion of the genetic diversity available in C. canephora, the Timor Hybrid obviously constitutes a considerable source of genetic diversity for arabica breeding. Analysis of genetic relationships among the Timor Hybrid-derived genotypes suggested that introgression was not restricted to chromosome substitution but also involved chromosome recombinations. Furthermore, the Timor Hybrid-derived genotypes varied considerably in the number of AFLP markers attributable to introgression. In this way, the introgressed markers identified in the analysed arabica coffee introgressed genotypes were estimated to represent from 9% to 29% of the C. canephora genome. Nevertheless, the amount of alien genetic material in the introgression arabica lines remains substantial and should justify the development of adapted breeding strategies. Springer-Verlag Berlin Heidelberg, 2000 Key words Coffea arabica nationallicence Introgression nationallicence Genetic diversity nationallicence Alien gene transfer nationallicence AFLP nationallicence Lashermes P. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut Andrzejewski S. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut Bertrand B. IICA/PROMECAFE, Ap. Postal 55, 2200, Coronado, San José, Costa Rica, CR aut Combes M. C. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut Dussert S. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut Graziosi G. Universitá deli Studi di Trieste, I-34148, Trieste, Italy, IT aut Trouslot P. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut Anthony F. CATIE, AP59, 7170 Turrialba, Costa Rica, CR aut https://doi.org/10.1007/s001220050019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lashermes P. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut NATIONALLICENCE 700 1- Andrzejewski S. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut NATIONALLICENCE 700 1- Bertrand B. IICA/PROMECAFE, Ap. Postal 55, 2200, Coronado, San José, Costa Rica, CR aut NATIONALLICENCE 700 1- Combes M. C. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut NATIONALLICENCE 700 1- Dussert S. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut NATIONALLICENCE 700 1- Graziosi G. Universitá deli Studi di Trieste, I-34148, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Trouslot P. IRD (ex ORSTOM), GeneTrop, BP 5045, F-34032, Montpellier, France e-mail: Philippe.Lashermes@mpl.ird.fr Fax: +33-4-67-54-78-00, FR aut NATIONALLICENCE 700 1- Anthony F. CATIE, AP59, 7170 Turrialba, Costa Rica, CR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer