caa a22 4500 475795717 CHVBK 20180406123723.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s001220050012 doi (NATIONALLICENCE)springer-10.1007/s001220050012 Novel proteinase inhibitors in seeds of sunflower ( Helianthus annuus L.): polymorphism, inheritance and properties [Elektronische Daten] [Al. V. Konarev, I. N. Anisimova, V. A. Gavrilova, V. T. Rozhkova, R. Fido, A. S. Tatham, P. R. Shewry] Abstract : A highly sensitive gelatin overlay procedure was used to identify inhibitors of serine proteinases and of the cysteine proteinase ficin in seeds and leaves of sunflower. One major and two minor groups of trypsin inhibitors were identified in seeds, the former having a high pI (@10) and also inhibiting chymotrypsin. Three groups of trypsin/subtilisin inhibitors were also present in seeds, together with three inhibitors of ficin. All groups showed polymorphism between lines of Helianthus annuus, while the trypsin and trypsin/subtilisin inhibitors also varied between wild species of Helianthus, with no apparent relationship to growth type (annual or perennial), genome constitution or ploidy level. Genetic analysis showed that the major trypsin inhibitor and three groups of trypsin/subtilisin inhibitors are each controlled by single Mendelian loci, with the three loci for trypsin/subtilisin inhibitors showing recombination values of 0.23-0.40. Purification by RP-HPLC allowed the M r of two trypsin inhibitors to be determined by SDS-PAGE to be about 1,500 and 2,500, while the three trypsin/subtilisin inhibitors varied in M r from about 1,500 to 6,000. Springer-Verlag Berlin Heidelberg, 2000 Key words Sunflower nationallicence Seeds nationallicence Proteinase inhibitors nationallicence Polymorphism nationallicence Genetic control nationallicence Konarev Al. V. All-Russian Institute of Plant Protection, St. Petersburg, Russia, RU aut Anisimova I. N. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut Gavrilova V. A. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut Rozhkova V. T. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut Fido R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut Tatham A. S. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut Shewry P. R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut https://doi.org/10.1007/s001220050012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001220050012 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Konarev Al. V. All-Russian Institute of Plant Protection, St. Petersburg, Russia, RU aut NATIONALLICENCE 700 1- Anisimova I. N. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut NATIONALLICENCE 700 1- Gavrilova V. A. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut NATIONALLICENCE 700 1- Rozhkova V. T. N.I. Vavilov All-Russian Institute of Plant Industry (VIR), St. Petersburg, Russia, RU aut NATIONALLICENCE 700 1- Fido R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut NATIONALLICENCE 700 1- Tatham A. S. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut NATIONALLICENCE 700 1- Shewry P. R. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, UK e-mail: Peter.Shewry@bbsrc.ac.uk, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer